Overview
- Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
- DescriptionRabbit polyclonal to Cdk9
- Tested applicationsICC/IF, Sandwich ELISA, IHC-P, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Cdk9.
(Peptide available as ab39096.)
- Positive controlThis antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line)
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab38840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| Sandwich ELISA | sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal to Cdk9 (ab76873). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal to Cdk9 (ab76873) as Capture. |
| IHC-P | IHC-P: Use a concentration of 1 µg/ml. |
| WB | WB: 1/250. Detects a band of approximately 41 kDa (predicted molecular weight: 43, 50 kDa).Can be blocked with Cdk9 peptide (ab39096) or Cdk9 peptide (ab39097). |
Target
- FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 1025 Human
- Entrez Gene: 1025 Human
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: P50750 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
see all
Target information above from: UniProt accession
P50750
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- C 2k antibodyC 2k antibodyC-2K antibody
- CDC2 related kinase antibodyCDC2 related kinase antibodyCDC2L4 antibodyCDC2L4 antibodyCdk 9 antibodyCdk9 antibodyCDK9_HUMAN antibodyCell division cycle 2 like protein kinase 4 antibodyCell division cycle 2-like protein kinase 4 antibodyCell division protein kinase 9 antibodyCell division protein kinase 9 antibodyCTK1 antibodyCyclin dependent kinase 9 antibodyCyclin dependent kinase 9 antibodyCyclin-dependent kinase 9 antibodyPITALRE antibodySerine threonine protein kinase PITALRE antibodySerine threonine protein kinase PITALRE antibodySerine/threonine-protein kinase PITALRE antibodyTAK antibodyTAK antibodyTat associated kinase complex catalytic subunit antibody
see all
Anti-Cdk9 antibody images
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Sandwich ELISA - Cdk9 antibody (ab38840)Standard Curve for Cdk9 (Analyte: Cdk9 protein (Tagged) (ab85603)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to Cdk9 (ab76873) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk9 (ab38840) at 0.5µg/ml. -
Western blot - Cdk9 antibody (ab38840)All lanes : Anti-Cdk9 antibody (ab38840) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 :A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lane 4 :HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 43, 50 kDa
Observed band size : 41 kDa (why is the actual band size different from the predicted?)
Additional bands at : 140 kDa,260 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Cdk9 antibody (ab38840)ICC/IF image of ab38840 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38840, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk9 antibody (ab38840)IHC image of ab38840 staining Cdk9 in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38840, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
References for Anti-Cdk9 antibody (ab38840)
ab38840 has not yet been referenced specifically in any publications.
