• Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
  • Description
    Rabbit polyclonal to Cdk9
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Cdk9.

    (Peptide available as ab39097.)

  • Positive control
    • This antibody gives a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) HEK 293 (Human embryonic kidney cell line)



Our Abpromise guarantee covers the use of ab39098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human Cdk9 peptide (ab39097).


  • FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C 2k antibody
    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2 like protein kinase 4 antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine threonine protein kinase PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Anti-Cdk9 antibody images

  • All lanes : Anti-Cdk9 antibody (ab39098) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab39098 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39098, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Anti-Cdk9 antibody (ab39098) at 1/250 dilution + Xenopus laevis gastrula embryo extract (Stage12) at 0.5 µg

    Goat Anti-rabbit IgG Alexa Fluor 680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa

    Exposure time : 5 minutes

    See Abreview

References for Anti-Cdk9 antibody (ab39098)

ab39098 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
Loading amount 0.5 µg
Specification Xenopus laevis gastrula embryo extract (Stage12)
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C

Dr. Kei Miyamoto

Verified customer

Submitted Oct 10 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Caenorhabditis elegans Cell (Intestinal Nuclei)
Specification Intestinal Nuclei
Fixative Paraformaldehyde
Permeabilization Yes - Sperm Salts Buffer, Triton X-100

Abcam user community

Verified customer

Submitted Jun 03 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Feb 25 2009