Overview

  • Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
  • Description
    Rabbit polyclonal to Cdk9
  • Tested applicationsICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Cdk9.

    (Peptide available as ab39097.)

  • Positive control
    • This antibody gives a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) HEK 293 (Human embryonic kidney cell line)

Properties

Applications

Our Abpromise guarantee covers the use of ab39098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/250. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human Cdk9 peptide (ab39097).

Target

  • FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C 2k antibody
    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2 like protein kinase 4 antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine threonine protein kinase PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Anti-Cdk9 antibody images

  • All lanes : Anti-Cdk9 antibody (ab39098) at 1/250 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab39098 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39098, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • Anti-Cdk9 antibody (ab39098) at 1/250 dilution + Xenopus laevis gastrula embryo extract (Stage12) at 0.5 µg

    Secondary
    Goat Anti-rabbit IgG Alexa Fluor 680 at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa


    Exposure time : 5 minutes

    See Abreview

References for Anti-Cdk9 antibody (ab39098)

ab39098 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Xenopus laevis gastrula embryo extract (Stage12))
Loading amount 0.5 µg
Specification Xenopus laevis gastrula embryo extract (Stage12)
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Dr. Kei Miyamoto

Verified customer

Submitted Oct 10 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Caenorhabditis elegans Cell (Intestinal Nuclei)
Specification Intestinal Nuclei
Fixative Paraformaldehyde
Permeabilization Yes - Sperm Salts Buffer, Triton X-100
Username

Abcam user community

Verified customer

Submitted Jun 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 25 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"