Anti-Cdk9 antibody (ab39098)

Overview

• Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
• Description
  Rabbit polyclonal to Cdk9
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human, Xenopus laevis
  Predicted to work with: Mouse, Rat, Chicken, Cow
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Cdk9.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab39097.)

• Positive controlThis antibody gives a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) HEK 293 (Human embryonic kidney cell line)

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Microbiology
  • Protein
  • Human Protein
  • Defensin

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling
  • HIV-related

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P50750 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1025 Human
  • Entrez Gene: 1025 Human
  • Entrez Gene: 1025 Human
  • Entrez Gene: 107951 Mouse
  • Entrez Gene: 362110 Rat
  • Omim: 603251 Human
  • SwissProt: P50750 Human
  • SwissProt: P50750 Human

  • SwissProt: P50750 Human
  • SwissProt: Q99J95 Mouse
  • SwissProt: Q641Z4 Rat
  • Unigene: 150423 Human
  • Unigene: 706809 Human
  • Unigene: 27557 Mouse
  • Unigene: 98228 Rat

  see all

• Alternative names
  C 2k antibodyC-2K antibodyCDC2 related kinase antibody

  CDC2L4 antibodyCdk 9 antibodyCdk9 antibodyCDK9_HUMAN antibodyCell division cycle 2 like protein kinase 4 antibodyCell division cycle 2-like protein kinase 4 antibodyCell division protein kinase 9 antibodyCTK1 antibodyCyclin dependent kinase 9 antibodyCyclin-dependent kinase 9 antibodyPITALRE antibodySerine threonine protein kinase PITALRE antibodySerine/threonine-protein kinase PITALRE antibodyTAK antibodyTat associated kinase complex catalytic subunit antibody

  see all

Anti-Cdk9 antibody images

• 

  Western blot - Cdk9 antibody (ab39098)
  All lanes : Anti-Cdk9 antibody (ab39098) at 1/250 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
  Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
  Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate (ab7902)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 43 kDa
  Observed band size : 43 kDa
  Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - Cdk9 antibody (ab39098)
  ICC/IF image of ab39098 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39098, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

• 

  Western blot - Anti-Cdk9 antibody (ab39098)
  Anti-Cdk9 antibody (ab39098) at 1/250 dilution + Xenopus laevis gastrula embryo extract (Stage12) at 0.5 µg
  
  Secondary
  Goat Anti-rabbit IgG Alexa Fluor 680 at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 43 kDa
  
  
  Exposure time : 5 minutes

  See Abreview