Anti-Cdk9 antibody (ab6544)
- Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
- DescriptionRabbit polyclonal to Cdk9
- SpecificityNo reaction is observed against other related cyclin dependent kinases.
- Tested applicationsICC/IF, ELISA, IHC-P, IP, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)
- General notescdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate
- PurityWhole antiserum
- Purification notesSterile filtered.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab6544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent concentration. PubMed: 21378166|
|ELISA||ELISA: 1/500 - 1/2000.|
|IHC-P||IHC-P: 1/200 - 1/1000.|
|IP||IP: Use at an assay dependent dilution.|
|WB||WB: 1/500 - 1/1000. Predicted molecular weight: 43 kDa.|
- FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
- Cellular localizationNucleus.
- C 2k antibody
- C-2K antibody
- CDC2 related kinase antibody
- CDC2L4 antibody
- Cdk 9 antibody
- Cdk9 antibody
- CDK9_HUMAN antibody
- Cell division cycle 2 like protein kinase 4 antibody
- Cell division cycle 2-like protein kinase 4 antibody
- Cell division protein kinase 9 antibody
- CTK1 antibody
- Cyclin dependent kinase 9 antibody
- Cyclin-dependent kinase 9 antibody
- PITALRE antibody
- Serine threonine protein kinase PITALRE antibody
- Serine/threonine-protein kinase PITALRE antibody
- TAK antibody
- Tat associated kinase complex catalytic subunit antibody
Anti-Cdk9 antibody images
Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.
Immunofluorescence analysis of HeLa cells, staining Cdk9 (red) with ab6544.
The cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 10 min at 4°C. The cells were incubated with 0.05% PBS-T before incubating with primary antibody at 1/2000 dilution.
References for Anti-Cdk9 antibody (ab6544)
This product has been referenced in:
- Zhang H et al. SIRT2 directs the replication stress response through CDK9 deacetylation. Proc Natl Acad Sci U S A 110:13546-51 (2013). Read more (PubMed: 23898190) »
- Qi T et al. G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb). J Biol Chem 286:15171-81 (2011). ICC/IF ; Human . Read more (PubMed: 21378166) »