Overview
- Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
- DescriptionRabbit polyclonal to Cdk9
- SpecificityNo reaction is observed against other related cyclin dependent kinases.
- Tested applicationsICC/IF, ELISA, IHC-P, IP, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Multiple synthetic peptides corresponding to C-terminal and N-terminal domains of the protein coded by the human gene cdk9 (PITALRE)
- General notescdk9(PITALRE) interacts with a conserved domain in the TRAF-C region of the tumor necrosis factor signal transducer TRAF2. The positive transcription elongation factor b (P-TEFb) is identified as cdk9 paired with cyclin T1.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
- PurityWhole antiserum
- Purification notesSterile filtered.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 21378166 |
| ELISA | ELISA: 1/500 - 1/2000. |
| IHC-P | IHC-P: 1/200 - 1/1000. |
| IP | IP: Use at an assay dependent dilution. |
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 43 kDa. |
Target
- FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 1025 Human
- Entrez Gene: 1025 Human
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: P50750 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
see all
Target information above from: UniProt accession
P50750
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- C 2k antibodyC-2K antibodyCDC2 related kinase antibody
- CDC2L4 antibodyCdk 9 antibodyCdk9 antibodyCDK9_HUMAN antibodyCell division cycle 2 like protein kinase 4 antibodyCell division cycle 2-like protein kinase 4 antibodyCell division protein kinase 9 antibodyCTK1 antibodyCyclin dependent kinase 9 antibodyCyclin-dependent kinase 9 antibodyPITALRE antibodySerine threonine protein kinase PITALRE antibodySerine/threonine-protein kinase PITALRE antibodyTAK antibodyTat associated kinase complex catalytic subunit antibody
see all
Anti-Cdk9 antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cdk9 antibody (ab6544)Immunohistochemical staining of mouse tissue using anti-cdk9 (PITALRE) antiserum. The staining shows the location of cdk9 / PITALRE protein in developing mouse tissue. Arrow indicates area of high expression.
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Immunocytochemistry/ Immunofluorescence - Anti-Cdk9 antibody (ab6544)Image from Qi T et al., J Biol Chem, 286, 15171-81. Fig 3.; doi: 10.1074/jbc.M110.184374. Epub 2011 Mar 4.Immunofluorescence analysis of HeLa cells, staining Cdk9 (red) with ab6544.
The cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 10 min at 4°C. The cells were incubated with 0.05% PBS-T before incubating with primary antibody at 1/2000 dilution.
References for Anti-Cdk9 antibody (ab6544)
This product has been referenced in:
- Qi T et al. G-actin participates in RNA polymerase II-dependent transcription elongation by recruiting positive transcription elongation factor b (P-TEFb). J Biol Chem 286:15171-81 (2011). ICC/IF ; Human . Read more (PubMed: 21378166) »
- Kruse U et al. Chemoproteomics-based kinome profiling and target deconvolution of clinical multi-kinase inhibitors in primary chronic lymphocytic leukemia cells. Leukemia : (2010). Read more (PubMed: 20944678) »
