Overview

  • Product nameAnti-Cdk9 antibody
    See all Cdk9 primary antibodies
  • Description
    Mouse monoclonal to Cdk9
  • Tested applicationsSandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Macaque Monkey
  • Immunogen

    GST-tagged recombinant fragment: QKRKVKDRLK AYVRDPYALD LIDKLLVLDP AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP ATTNQTEFER VF, corresponding to C terminal amino acids 271-372 of Human Cdk9 (AAH01968)

  • Positive control
    • Cdk9-transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cdk9 (ab38840). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Cdk9 (ab38840) as Detection.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged CDK9 is approximately 1ng/ml when used as a capture antibody.

Target

  • FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C 2k antibody
    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2 like protein kinase 4 antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • MGC95175 antibody
    • OTTHUMP0000002219 antibody
    • PITALRE antibody
    • RP11-228B15.5 antibody
    • Serine threonine protein kinase PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Anti-Cdk9 antibody images

Protocols

References for Anti-Cdk9 antibody (ab76873)

ab76873 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"