Overview
- Product nameAnti-Cdk9 antibodySee all Cdk9 primary antibodies ...
- DescriptionMouse monoclonal to Cdk9
- Tested applicationsSandwich ELISA, WB, ELISA more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Macaque Monkey - Immunogen
GST-tagged recombinant fragment: QKRKVKDRLK AYVRDPYALD LIDKLLVLDP AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP ATTNQTEFER VF, corresponding to C terminal amino acids 271-372 of Human Cdk9 (AAH01968)
- Positive controlCdk9-transfected 293T cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- IsotypeIgG2a
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Cdk9 (ab38840). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Cdk9 (ab38840) as Detection. |
| WB | WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 43 kDa. |
| ELISA | ELISA: Use at an assay dependent dilution. Detection limit for recombinant tagged CDK9 is approximately 1ng/ml when used as a capture antibody. |
Target
- FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
- Tissue specificityUbiquitous.
- Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 1025 Human
- Entrez Gene: 1025 Human
- Entrez Gene: 1025 Human
- Entrez Gene: 107951 Mouse
- Entrez Gene: 362110 Rat
- Omim: 603251 Human
- SwissProt: P50750 Human
- SwissProt: P50750 Human
- SwissProt: P50750 Human
- SwissProt: Q99J95 Mouse
- SwissProt: Q641Z4 Rat
- Unigene: 150423 Human
- Unigene: 706809 Human
- Unigene: 27557 Mouse
- Unigene: 98228 Rat
see all
Target information above from: UniProt accession
P50750
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- C 2k antibodyC-2K antibodyCDC2 related kinase antibody
- CDC2L4 antibodyCdk 9 antibodyCdk9 antibodyCDK9_HUMAN antibodyCell division cycle 2 like protein kinase 4 antibodyCell division cycle 2-like protein kinase 4 antibodyCell division protein kinase 9 antibodyCTK1 antibodyCTK1 antibodyCyclin dependent kinase 9 antibodyCyclin-dependent kinase 9 antibodyMGC95175 antibodyOTTHUMP0000002219 antibodyPITALRE antibodyRP11-228B15.5 antibodySerine threonine protein kinase PITALRE antibodySerine/threonine-protein kinase PITALRE antibodyTAK antibodyTat associated kinase complex catalytic subunit antibody
see all
Anti-Cdk9 antibody images
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Sandwich ELISA - Cdk9 antibody (ab76873)Standard Curve for Cdk9 (Analyte: Cdk9 protein (Tagged) (ab85603)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to Cdk9 (ab76873) at 5µg/ml and Detector Antibody Rabbit polyclonal to Cdk9 (ab38840) at 0.5µg/ml. -
Western blot - Cdk9 antibody (ab76873)Anti-Cdk9 antibody (ab76873) at 1 µg/ml + immunogen (GST-tagged 102aa recombinant fragment) at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution
Predicted band size : 43 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?) -
Western blot - Cdk9 antibody (ab76873)All lanes : Anti-Cdk9 antibody (ab76873) at 1 µg/ml
Lane 1 : Cdk9-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Cdk9 antibody (ab76873)
ab76873 has not yet been referenced specifically in any publications.
