Anti-Cdk9 (phospho T186) antibody (ab79178)

Overview

  • Product name
    Anti-Cdk9 (phospho T186) antibody
    See all Cdk9 primary antibodies
  • Description
    Rabbit polyclonal to Cdk9 (phospho T186)
  • Specificity
    ab79178 detects endogenous levels of CDK9 only when phosphorylated at threonine 186.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Cdk9 around the phosphorylation site of threonine 186 (R-Y-TP-N-R).

  • Positive control
    • 3T3 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab79178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
ELISA 1/20000.

Target

  • Function
    Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-2K antibody
    • CDC2 related kinase antibody
    • CDC2L4 antibody
    • Cdk 9 antibody
    • Cdk9 antibody
    • CDK9_HUMAN antibody
    • Cell division cycle 2-like protein kinase 4 antibody
    • Cell division protein kinase 9 antibody
    • CTK1 antibody
    • Cyclin dependent kinase 9 antibody
    • Cyclin-dependent kinase 9 antibody
    • PITALRE antibody
    • Serine/threonine-protein kinase PITALRE antibody
    • TAK antibody
    • Tat associated kinase complex catalytic subunit antibody
    see all

Images

  • All lanes : Anti-Cdk9 (phospho T186) antibody (ab79178)

    Lane 1 : 3T3 cells treated with Forskolin (40 nM, 30 minutes)
    Lane 2 : 3T3 cells treated with Forskolin (40 nM, 30 minutes) with phospho peptide


    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References

ab79178 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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