Anti-Cdk9 (phospho T186) antibody (ab79178)

Overview

• Product nameAnti-Cdk9 (phospho T186) antibodySee all Cdk9 primary antibodies ...
• Description
  Rabbit polyclonal to Cdk9 (phospho T186)
• Specificityab79178 detects endogenous levels of CDK9 only when phosphorylated at threonine 186.
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat
• Immunogen

  Synthesized phosphopeptide derived from human Cdk9 around the phosphorylation site of threonine 186 (R-Y-T^P-N-R).

• Positive control3T3 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Microbiology
  • Protein
  • Human Protein
  • Defensin

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Kinases/Phosphatases
  • Cdks

  • Immunology
  • Immune System Diseases
  • Antiviral Signaling
  • HIV-related

  • Cell Biology
  • Cell Cycle
  • Kinases/Phosphatases
  • Cdks

Applications

Target

• FunctionMember of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
  Contains 1 protein kinase domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P50750 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1025 Human
  • Entrez Gene: 1025 Human
  • Entrez Gene: 1025 Human
  • Entrez Gene: 107951 Mouse
  • Entrez Gene: 362110 Rat
  • Omim: 603251 Human
  • SwissProt: P50750 Human
  • SwissProt: P50750 Human

  • SwissProt: P50750 Human
  • SwissProt: Q99J95 Mouse
  • SwissProt: Q641Z4 Rat
  • Unigene: 150423 Human
  • Unigene: 706809 Human
  • Unigene: 27557 Mouse
  • Unigene: 98228 Rat

  see all

• Alternative names
  C 2k antibodyC-2K antibodyCDC2 related kinase antibody

  CDC2L4 antibodyCdk 9 antibodyCdk9 antibodyCDK9_HUMAN antibodyCell division cycle 2 like protein kinase 4 antibodyCell division cycle 2-like protein kinase 4 antibodyCell division protein kinase 9 antibodyCTK1 antibodyCTK1 antibodyCyclin dependent kinase 9 antibodyCyclin-dependent kinase 9 antibodyMGC95175 antibodyOTTHUMP0000002219 antibodyPITALRE antibodyRP11-228B15.5 antibodySerine threonine protein kinase PITALRE antibodySerine/threonine-protein kinase PITALRE antibodyTAK antibodyTat associated kinase complex catalytic subunit antibody

  see all

Anti-Cdk9 (phospho T186) antibody images

• 

  Western blot - Cdk9 (phospho T186) antibody (ab79178)
  All lanes : Anti-Cdk9 (phospho T186) antibody (ab79178)
  
  Lane 1 : 3T3 cells treated with Forskolin (40 nM, 30 minutes)
  Lane 2 : 3T3 cells treated with Forskolin (40 nM, 30 minutes) with phospho peptide
  
  
  Predicted band size : 43 kDa
  Observed band size : 43 kDa