Cell Fractionation Kit - HT (ab109718)
- Product nameCell Fractionation Kit - HTSee all Cell Fractionation kits ...
- Tests1 x 96 well plate
- Assay typeDirect
- Assay time1h 00m
- Species reactivityReacts with: Human, a wide range of other species
- Product overview
ab109718 (MS862) provides a method and reagents for a simple and rapid preparation of cytosolic, mitochondrial and nuclear fractions from adherent cells. The kit is especially suitable for, but not limited to, high throughput fractionation of adherent cells in a 96-well plate format. The entire fractionation of 96 samples into the cytosolic, mitochondrial and nuclear fraction is achieved in one hour. The kit is based on sequential detergent extraction of cytosolic, mitochondrial and nuclear proteins without the need for mechanical disruption of cells, and thus fractionates adherent cells into cytosol-containing, mitochondria-containing and nuclei-containing fractions. These fractions are referred throughout the protocol as cytosolic, mitochondrial and nuclear fractions. The kit prepares sufficient sample material for subsequent Western blot analyses, dot blot analyses or for analyses by microplate ELISA assays.
ab109718 is designed to allow the measurement of any proteins which are differentially represented in the cytosol, mitochondria and nuclei, and is particularly applicable to studies of proteins that translocate between these three cellular compartments.
A second version of the kit is also available, ab109719 (MS861) Cell Fractionation Kit - Standard. This version is designed for fractionating cells grown in batches and is ideal for experiments requiring larger sample amounts.
Preparation of cytosolic, mitochondrial and nuclear fractions in microplate format.
Storage: Store Buffer A, Detergent HT II, Detergent HT III and 5X SDS Sample Buffer at -20°C. Store Detergent HT I at -80°C. Store the collection plates and seal films at room temperature.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 x 96 tests 5X SDS Sample Buffer 1 x 1.5ml 96-well collection plate with seal film 3 units Buffer A 1 x 34ml Detergent HT I 1 x 7.5µl Detergent HT II 1 x 35µl Detergent HT III 1 x 700µl
- Research Areas
Our Abpromise guarantee covers the use of ab109718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Cell Fractionation Kit - HT (ab109718)
This product has been referenced in:
- Poster presented at the combined Keystone Symposia Cell Death Pathways and Mitochondrial Dynamics and Physiology, March 22-27, 2009. <a href="http://www.mitosciences.com/PDF/keystone_poster.pdf">Download</a> : (0). Read more (PubMed: ) »