Cell Fractionation Kit Standard (ab109719)
- Product nameCell Fractionation Kit StandardSee all Cell Fractionation kits ...
- Assay typeDirect
- Species reactivityReacts with: Human, a wide range of other species
- Product overview
ab109719 (MS861) allows for the rapid and simple preparation of mitochondrial, cytoplasmic and nuclear fractions from cultured cells. Unlike competing products, this kit does not require mechanical disruption of the sample. This is an important performance characteristic, as mechanical disruption can also disrupt mitochondrial membranes, causing the biologically-irrelevant release of proteins. Particularly useful in studying apoptosis and the movement of pro-apoptotic proteins such as cytochrome c.
A companion product is also available which allows for the fractionation of cells grown in 96-well plates: Cell Fractionation Kit HT (ab109718/MS862).
- Storage instructionsPlease refer to protocols.
Components 1 kit 5 X SDS Sample Buffer 1 x 10ml Buffer A 2x concentrate 1 x 175ml Detergent I 1 x 25µl Detergent II 1 x 1ml
- Research Areas
Cell Fractionation Kit Standard images
In this experiment, apoptosis was induced in Jurkat, HeLa and 143B osteosarcoma cells by treatment with staurosporine or in Jurkat cells by FAS. Mitochondrial and cytoplasmic fractions were isolated (using ab109719) and probed using ab110415 (MSA12). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.
References for Cell Fractionation Kit Standard (ab109719)
This product has been referenced in:
- Sanchez-Alvarez R et al. Mitochondrial dysfunction in breast cancer cells prevents tumor growth: understanding chemoprevention with metformin. Cell Cycle 12:172-82 (2013). Read more (PubMed: 23257779) »
- Ameri K et al. Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress. PLoS One 8:e62758 (2013). Mouse . Read more (PubMed: 23646141) »