Cell Proliferation Assay Kit (Fluorometric - Blue) (ab102501)

Overview

  • Product nameCell Proliferation Assay Kit (Fluorometric - Blue)See all Cell proliferation kits ...
  • Tests
    500 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Sensitivity
    > 100 cells/well
  • Range
    100 cells/well - 100000 cells/well
  • Product overview

    Abcam's Cell Proliferation Assay Kit (Fluorometric-Blue) provides by far the most sensitive and easiest means for quantifying cell proliferation and viability. Simply add the single reagent to cell culture, incubate and read the fluorescence intensity. The assay utilizes the redox dye resazurin which is not fluorescent, but upon reduction by viable metabolically active cells, the dye becomes highly fluorescent (Ex = 530-570 nm; Em = 590-620 nm). Therefore, viable metabolically active cells can be easily measured.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Key Features:

    1. Simple procedure: Just Add-Incubate-Read. No washing, No solublization.
    2. Highly Sensitive: Detect as few as 100 cells.
    3. Longer Stability: Reagent is stable at 4C for at least a year.
    4. Safe: Non-radioactive, non-toxic, and cells can be further used for other experiments.
    5. Wide applications: Used in studying a variety of growth stimulations or inhibitions (e.g., by growth factors, cytokine, nutrients, anticancer drugs, apoptosis inducers/inhibitors, toxicityinducing chemicals, etc.).

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population.

Applications

Our Abpromise guarantee covers the use of ab102501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Functional Studies Use at an assay dependent dilution.

Cell Proliferation Assay Kit (Fluorometric - Blue) images

Protocols

References for Cell Proliferation Assay Kit (Fluorometric - Blue) (ab102501)

ab102501 has not yet been referenced specifically in any publications.

Product Wall

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Unfortunately, we do not have a kit which combines these two factors in one, or more precisely: we haven't tested them in combination. However, we do have an abundance of kits which can measure this two factors s...

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I don't think this should be a problem, but it may be necessary to increase the the dilution factor for your sample to avoid that the measurements are outside the scale.
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This kit provides sufficient reagents for 5 96-well microtiter plates (500 assays).

The kit is similar to MTT assays in that it is designed to measure cell proliferation. However it is simpler and more se...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"