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Cell Viability Assay Kit (Bioluminescent) (ab65314)

Q&A (8)

Overview

  • Product nameCell Viability Assay Kit (Bioluminescent)See all Cell Viability kits ...
  • Tests
    200 x 1 assay
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • Assay typeDirect
  • Sensitivity
    > 10 cells/well
  • Range
    10 cells/well - 100 cells/well
  • Assay time
    0h 30m
  • Product overview

    Abcam's Cell Viability Assay Kit (Bioluminescent) utilizes bioluminescent detection of the ATP levels for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample). The high sensitivity of this assay has led to many other applications for detecting ATP production in various enzymatic reactions, as well as for detecting low level bacterial contamination in samples such as blood, milk, urine, soil, and sludge.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Cell death (especially apoptosis) is an energy-dependent process that requires ATP. As ATP levels fall to a point where the cell can no longer perform basic metabolic functions, the cell will die. A typical apoptotic cell exhibits a significant decrease in ATP level. Therefore, loss of ATP level in cell has been used as an indicator of cell death. In contrast, cell proliferation has been recognized by increased levels of ATP.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.

    • Applications

    Our Abpromise guarantee covers the use of ab65314 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Protocols

    References for Cell Viability Assay Kit (Bioluminescent) (ab65314)

    ab65314 has not yet been referenced specifically in any publications.

    Publishing research using ab65314 ? Please let us know so that we can cite the reference in this datasheet

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    Displaying 1 - 8 of 8 results for Abreviews and Q&A

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    I want to test for viability in cell culture supernatants. We are using RPMI + 10% FBS in our assay. Will this have an effect in the readout?



    I've confirmed with the lab, and you can use this kind of media but be sure to use a control media with the same amount of RPMI and FBS to account for any signals generated due to these 2 in your sample readings. Just subtract the control readin...

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    I want to test for viability in cell culture supernatants. We are using RPMI + 10% FBS in our assay. Will this have an effect in the readout?



    abcam.com

    Hello!

    You can use a control media with the same amount of RPMI and FBS to account for any signals generated due to these 2 in your sample readings. Just subtract the readings you get in the controls from the standards.

    Please let me kno...

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    I see that the readout would be ATP in the media; this means that non-viable (or dead) cells would have lower ATP readings in the media compared to live cells.


    Yes, you are correct. Lower ATP readings would indicate a decrease in cell viability.

    Can you please suggest a product for which I can test cell viability using cell supernatants?

    Ab65314 is recommended for cell viability. For media, use the same protocol as mentioned for the suspension cells in step 2.a.


    I have one question regarding the cell viability assay kit (ab65314).
    In the manual is not very clear to me if each sample should be read for 1 minute or not. I found 2 different indications:

    The assay can be fully automatic for high ...

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    Thank you for contacting us and for asking your question about the Cell Viability Assay Kit instructions.

    The instruction on page 7 of the protocol to read the samples for one minute after adding the enzyme is mistaken. It should read:
    Read More

    Sure you suggest a luminometer but a beta counter as well in the protocol, for this reason I wrote you asking for more information about the Beta counter setup.

    Thank you for your reply. I can confirm that conventional scintillation counters can not be used for measurements with this kit. However, when Beta Counter is used it should be programmed in the “out of coincidence” (or Luminescence mode) for meas...

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    Dear ABcam, concerning the protocol, I'd like to know if I can use a Beckman Coulter LS6500 Multipurpose Scintillation Counter to carry out my experiment and how i could setup my instrument. Thank you

    Thank you for your enquiry. I can confirm that the read out of ab65314  Cell Viability Assay Kit is via luminescence. Therefore, we would suggest to use the indicated equipment which is a luminometer.  Theoretically a luminometer and a scintillation cou...

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    I have a question concerning the ATP assay kit (ab65314. I work with isolated mitochondria (from mammalian tissue) and my question is whether your kit will work in my case.

    Thank you for your enquiry. I have contacted the originator of this kit who have confirmed that it can be used for measuring the ATP in mitochondrial lysates. Once you have produced your mitoochondria lysates, you can follow the kit protocol and get t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"