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There are a variety of parameters that can be used to monitor cell viability. The green fluorescent dye used in the kit is a hydrophobic compound. It easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly green fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The green fluorophore generated by the non-fluorescent substrate used in the kit has the spectral properties of fluorescein at Ex/Em = ~490 nm/520 nm. When well excited with the Argon Laser at 488 nm, the fluorophore emits intense green fluorescence at ~520 nm.
ab112122 provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays. It can also be used with a fluorescence microscope equipped with a FITC filter set.
ab112122 provides an effective tool of labeling cells for fluorescence microplate and microscopic investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112122 is suitable for proliferating and non-proliferating cells.
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|Components||5 x 96 tests|
|Assay Buffer||1 x 50ml|
|CellGreen fluorescent dye||5 vials|
|DMSO||1 x 200µl|
Our Abpromise guarantee covers the use of ab112122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab112122 has not yet been referenced specifically in any publications.