Cell Viability Assay Kit (Fluorometric - Green) (ab112122)

Overview

  • Product nameCell Viability Assay Kit (Fluorometric - Green)See all Cell Viability kits ...
  • Tests
    5 x 96 well plate
  • Sample type
    Adherent cells, Suspension cells
  • Species reactivity
    Reacts with: Human, Chinese Hamster
  • Product overview

    There are a variety of parameters that can be used to monitor cell viability. The green fluorescent dye used in the kit is a hydrophobic compound. It easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly green fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The green fluorophore generated by the non-fluorescent substrate used in the kit has the spectral properties of fluorescein at Ex/Em = ~490 nm/520 nm. When well excited with the Argon Laser at 488 nm, the fluorophore emits intense green fluorescence at ~520 nm.

    ab112122 provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays. It can also be used with a fluorescence microscope equipped with a FITC filter set.

    ab112122 provides an effective tool of labeling cells for fluorescence microplate and microscopic investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112122 is suitable for proliferating and non-proliferating cells.

    Visit our FAQs page for tips and troubleshooting.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceCell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.

Applications

Our Abpromise guarantee covers the use of ab112122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Cell Viability Assay Kit (Fluorometric - Green) images

  • CHO-K1 cell number response was measured with ab112122. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).

Protocols

References for Cell Viability Assay Kit (Fluorometric - Green) (ab112122)

ab112122 has not yet been referenced specifically in any publications.

Product Wall



Les filtres à utiliser sont FITC (vert)et Cy3ou Texas red (rouge).

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MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the ce...

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Thank you for contacting us regarding this issue. I have spoken with the lab about you results using ab112122. It seems from your results that most likely you have used the top read mode. For this assay, it must use bottom read mode for measuring, an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"