Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)
- Product nameCell Viability Assay Kit (Fluorometric - Near InfraRed)See all Cell Viability kits ...
- Sample typeAdherent cells, Suspension cells
- Assay typeDirect
- Product overview
Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. The proprietary far red fluorescent dye used in the kit is a hydrophobic compound that can easily permeate intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the weakly fluorescent substrate by intracellular esterases generates a strongly red fluorescent hydrophilic product that is well-retained in the cell cytoplasm. When well excited with the He-Ne Laser at 633 nm, the red fluorophore emits intense red fluorescence at ~660 nm.
ab112123 is an effective tool of labeling cells for the investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112123 is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. It comes with reagents sufficient to perform 200 assays in a 96-well plate or 800 assays in a 384-well plate. The kit provides all the essential components with an optimized protocol for microplate reader analysis of cell viability.
Visit our FAQs page for tips and troubleshooting.
ab112123 should be stored desiccated.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 2 x 96 tests Assay Buffer 2 x 10ml Cell NIR fluorescent dye 2 vials DMSO 1 x 100µl
- Research Areas
Our Abpromise guarantee covers the use of ab112123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cell Viability Assay Kit (Fluorometric - Near InfraRed) images
CHO-K1 cell number response measured with ab112123.
CHO-K1 cells at 0 to 50,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Near InfraRed Dye solution for 1 hour at room temperature. The fluorescence intensity was measured at Ex/Em = 635/670 nm.
References for Cell Viability Assay Kit (Fluorometric - Near InfraRed) (ab112123)
ab112123 has not yet been referenced specifically in any publications.