Overview

  • Product name
    Anti-Cell adhesion molecule 4 antibody
    See all Cell adhesion molecule 4 primary antibodies
  • Description
    Rabbit polyclonal to Cell adhesion molecule 4
  • Tested applications
    Suitable for: IP, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from an internal region of human Cell adhesion molecule 4.

  • Positive control
    • A549 cells, extracts from RAW264.7 cells, human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 21482674
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.
  • Tissue specificity
    Expressed in brain, prostate, brain, kidney and some other organs.
  • Sequence similarities
    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CADM 4 antibody
    • cadm4 antibody
    • CADM4_HUMAN antibody
    • Cell adhesion molecule 4 antibody
    • IGSF4C antibody
    • Immunoglobulin superfamily member 4C antibody
    • Necl 4 antibody
    • NECL-4 antibody
    • NECL4 antibody
    • Nectin like 4 antibody
    • Nectin like protein 4 antibody
    • Nectin-like protein 4 antibody
    • synCAM4 antibody
    • TSLC1 like 2 antibody
    • TSLC1 like protein 2 antibody
    • TSLC1-like protein 2 antibody
    • TSLL2 antibody
    see all

Images

  • All lanes : Anti-Cell adhesion molecule 4 antibody (ab69605) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells
    Lane 2 : extracts from RAW264.7 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • A549 cells,stained with ab69605 at 1/500-1/1000 dilution, with and without the immunising peptide.
  • Human brain tissue stained with ab69605 at 1/50-1/100 dilution, with and without the immunising peptide.

References

This product has been referenced in:
  • Sayar I  et al. Necl 4 and RNase 5 Are Important Biomarkers for Gastric and Colon Adenocarcinomas. Med Sci Monit 23:2654-2659 (2017). IHC-P ; Human . Read more (PubMed: 28561015) »
  • Roberts MD  et al. Nucleus accumbens neuronal maturation differences in young rats bred for low versus high voluntary running behaviour. J Physiol 592:2119-35 (2014). Rat . Read more (PubMed: 24665095) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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