Anti-Cellubrevin antibody (ab5789)
Key features and details
- Rabbit polyclonal to Cellubrevin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cellubrevin antibody
See all Cellubrevin primary antibodies -
Description
Rabbit polyclonal to Cellubrevin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse Cellubrevin aa 1-18.
Sequence:MSTGVPSGSSAATGSNRR
(Peptide available asab5861) -
Positive control
- WB: HeLa cell extract (ab7898) ; Mouse Brain ; Rat Brain ICC/IF: NIH/3T3 (Mouse embryonic fibroblast cell line) ; Neuro-2a (Mouse neuroblastoma cell line)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
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ICC/IF |
1/50.
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Notes |
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WB
1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa). |
ICC/IF
1/50. |
Target
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Function
SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. -
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 9341 Human
- Entrez Gene: 22319 Mouse
- Entrez Gene: 29528 Rat
- Omim: 603657 Human
- SwissProt: Q15836 Human
- SwissProt: P63024 Mouse
- SwissProt: P63025 Rat
- Unigene: 66708 Human
see all -
Alternative names
- CEB antibody
- Cellubrevin antibody
- Synaptobrevin 3 antibody
see all
Images
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All lanes : Anti-Cellubrevin antibody (ab5789)
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lane 3 : Mouse Liver
Lane 4 : Rat Liver
Predicted band size: 12 kDa
Additional bands at: ~15 kDa. We are unsure as to the identity of these extra bands.Relative expression of Cellubrevin observed in Mouse Brain and Rat Brain in comparison to Mouse Liver and Rat Liver using ab5789 to demonstrate specificty of expression of target across different tissues.
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Western blot analysis of recombinant rat VAMP-3 using ab5789.
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Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.
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Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, NIH/3T3 (Mouse embryonic fibroblast cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue). Actin was stained with Alexa Fluor® 555 (Red). The images were captured at 60X magnification.
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Immunofluorescent analysis of formalin-fixed, 0.1% Triton™ X-100 permeabilized, Neuro-2a (Mouse neuroblastoma cell line) cells, blocked with 3% BSA-PBS for 30 minutes at room temperature. Labelling cellubrevin with ab5789 at 1/50 dilution and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody at room temperature in the dark (right image). Compared with negative control without primary antibody (left image). Nuclei were stained with DAPI (Blue).The images were captured at 60X magnification.
Datasheets and documents
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Datasheet download
References (5)
ab5789 has been referenced in 5 publications.
- Lasic E et al. Astrocyte Specific Remodeling of Plasmalemmal Cholesterol Composition by Ketamine Indicates a New Mechanism of Antidepressant Action. Sci Rep 9:10957 (2019). PubMed: 31358895
- Habtemichael EN et al. Usp25m protease regulates ubiquitin-like processing of TUG proteins to control GLUT4 glucose transporter translocation in adipocytes. J Biol Chem 293:10466-10486 (2018). PubMed: 29773651
- Verboogen DRJ et al. Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules. FEBS Lett 592:1535-1544 (2018). PubMed: 29570778
- Verboogen DRJ et al. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife 6:N/A (2017). WB, ICC/IF ; Human . PubMed: 28524818
- Caceres PS et al. Vesicle-associated membrane protein 2 (VAMP2) but Not VAMP3 mediates cAMP-stimulated trafficking of the renal Na+-K+-2Cl- co-transporter NKCC2 in thick ascending limbs. J Biol Chem 289:23951-62 (2014). WB ; Rat . PubMed: 25008321