Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)
Key features and details
- Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cellular Apoptosis Susceptibility/CSE1L antibody
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies -
Description
Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Zebrafish -
Immunogen
Recombinant fragment corresponding to Human Cellular Apoptosis Susceptibility/CSE1L aa 222-439.
Database link: NP_001307 -
Positive control
- WB: HEK-293T, A431, H1299, HeLa whole cell lysate. PC-12 and Rat2 whole cell lysate. NIH/3T3, JC, BCL-1 and C2C12 whole cell lysate. IHC-P: Cal27 Xenograft. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/20000. Predicted molecular weight: 110 kDa.
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IHC-P |
1/100 - 1/2000.
Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH 8.0) |
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/20000. Predicted molecular weight: 110 kDa. |
IHC-P
1/100 - 1/2000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH 8.0) |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
Tissue specificity
Highly expressed in proliferating cells. -
Sequence similarities
Belongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 518622 Cow
- Entrez Gene: 1434 Human
- Entrez Gene: 110750 Mouse
- Entrez Gene: 362273 Rat
- Entrez Gene: 30707 Zebrafish
- Omim: 601342 Human
- SwissProt: P55060 Human
- SwissProt: Q9ERK4 Mouse
see all -
Alternative names
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
see all
Images
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HeLa cells stained for CSE1L (green) using ab96755 at 1/2000 dilution in ICC/IF.
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All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/10000 dilution
Lane 1 : PC-12 whole cell lysate
Lane 2 : Rat2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96755 at 1/500 dilution.
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All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa -
All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/10000 dilution
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa7.5% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab96755 has been referenced in 10 publications.
- Miyamoto Y et al. SARS-CoV-2 ORF6 disrupts nucleocytoplasmic trafficking to advance viral replication. Commun Biol 5:483 (2022). PubMed: 35590097
- Zhou S et al. FLVCR1 Predicts Poor Prognosis and Promotes Malignant Phenotype in Esophageal Squamous Cell Carcinoma via Upregulating CSE1L. Front Oncol 11:660955 (2021). PubMed: 33842377
- Schaff DL et al. Fragmentation of Small-Cell Lung Cancer Regulatory States in Heterotypic Microenvironments. Cancer Res 81:1853-1867 (2021). PubMed: 33531375
- Kalita J et al. On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions. J Cell Sci 134:N/A (2021). PubMed: 33912945
- Moriyama T et al. Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-a. Sci Rep 10:18640 (2020). PubMed: 33122699
- Hu X et al. LncRNA Oprm1 overexpression attenuates myocardial ischemia/reperfusion injury by increasing endogenous hydrogen sulfide via Oprm1/miR-30b-5p/CSE axis. Life Sci 254:117699 (2020). PubMed: 32437793
- Ma S et al. LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer. Aging (Albany NY) 10:2062-2078 (2018). PubMed: 30144787
- Tian C et al. Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol 24:1416-1427.e5 (2017). PubMed: 28988947
- Gagné B et al. Importin ß1 targeting by hepatitis C virus NS3/4A protein restricts IRF3 and NF-?B signaling of IFNB1 antiviral response. Traffic 18:362-377 (2017). PubMed: 28295920
- Sugiyama K et al. Calpain-Dependent Degradation of Nucleoporins Contributes to Motor Neuron Death in a Mouse Model of Chronic Excitotoxicity. J Neurosci 37:8830-8844 (2017). PubMed: 28821644