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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Centromeres
This fast track antibody is not yet fully characterised. It is subject to these terms and conditions
Overview
Product name
Anti-Cenexin1/ODF2 antibody
See all Cenexin1/ODF2 products (3) ...
Description
Rabbit polyclonal to Cenexin1/ODF2
This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
Specificity
On the basis of the immunogen sequence, we would expect this antibody to recognise the following isoforms of Cenexin1/ODF2 as listed in Swissprot: Q5BJF6-1 (95 kDa), Q5BJF6-2 (89 kDa), Q5BJF6-3 (93 kDa), and Q5BJF6-4 (95 kDa).
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human Cenexin1/ODF2.
(Peptide available as ab43839.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Reproduction >> Fertilisation
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Centromeres
Target
Function
Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
Tissue specificity
Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
Sequence similarities
Belongs to the ODF2 family.
Post-translational
modifications
Tyrosine phosphorylated.
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
Target information above from: UniProt accessionQ5BJF6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- sperm tail structural protein antibody
- 84 kDa outer dense fiber protein antibody
- Cancer/testis antigen 134 antibody
see all
Database links
- Entrez Gene: 4957 Human
- Entrez Gene: 18286 Mouse
- Omim: 602015 Human
- SwissProt: Q5BJF6 Human
- SwissProt: A3KGV1 Mouse
- Unigene: 129055 Human
- Unigene: 330116 Mouse
Anti-Cenexin1/ODF2 antibody images:
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Immunocytochemistry/ Immunofluorescence - Cenexin1/ODF2 antibody (ab43840)
ab43840 staining Cenexin1 in the mother centriole of a dividing HeLa cell by ICC/IF. DNA is stained blue, Aurora A is stained red, and Cenexin1 is stained green. Cells were fixed in formaldehyde, permeabilized with 0.1% Triton in PBS, and blocked with 2% BSA for 1 hour at 21°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 21°C. A FITC-conjugated anti-rabbit IgG H+L antibody was used as the secondary.
Image courtesy of an anonymous abreview
Western blot - Cenexin1/ODF2 antibody (ab43840)
All lanes : Anti-Cenexin1/ODF2 antibody (ab43840) at 1/500 dilution
Lane 1 : 25 ug HeLa cell lysate
Lane 2 : 12.5 ug HeLa cell lysate
Lane 3 : 25 ug HeLa cell lysate with Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Lane 4 : 12.5 ug HeLa cell lysate with Cenexin1/ODF2 peptide (ab43839) at 4 µg/ml
Secondary
Anti-Rabbit IgG HRP-conjugated at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 93 kDa
Observed band size : ~75 kDa (why is the actual band size different from the predicted?)
Exposure time : 4 minutes
Detection of Cenexin 1 by ab43840 in Western blot of HeLa cell lysates. Please see anonymous abreview for further details.
Image courtesy of an anonymous abreview.
References for Anti-Cenexin1/ODF2 antibody (ab43840)
ab43840 has not yet been referenced specifically in any publications.
Publishing research using ab43840? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"