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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Anti-Centaurin alpha 1 antibody (ab27476)
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab27476? Please let us know so that we can cite the reference in this datasheet
ab27476 has been referenced in 1 publications.
- Moore CD et al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P; Human, Rat. PubMed: 17635995
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Centaurin alpha 1 antibody (ab27476)
Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Centaurin alpha 1 antibody(ab27476)
IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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