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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-Centaurin alpha 1 antibody
See all Centaurin alpha 1 products (3) ...
Description
Goat polyclonal to Centaurin alpha 1
Tested applications
IHC-P, ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: CQEYAVEAHFKHKP, corresponding to amino acids 362-374 of Human Centaurin alpha 1.
CQEYAVEAHF KHKP
Positive control
Human and mouse brain lysates for Western blot.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: 1/32000.
ICC/IF: Use at an assay dependent dilution PMID 17635995.
IHC-P: Use at an assay dependent dilution PMID 17635995.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
Cellular localization
Cytoplasmic, Nuclear and Plasma membrane
Alternative names
- ArfGAP with dual PH domains 1 antibody
- CENTA 1 antibody
- CENTA1 antibody
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Database links
- Entrez Gene: 11033 Human
- Entrez Gene: 231821 Mouse
- Entrez Gene: 171097 Rat
- Omim: 608114 Human
- SwissProt: O75689 Human
- SwissProt: Q8BVR8 Mouse
- SwissProt: O88768 Rat
- Unigene: 135183 Human
see all
Anti-Centaurin alpha 1 antibody images:
Western blot - Centaurin alpha 1 antibody (ab27476)
Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Centaurin alpha 1 antibody(ab27476)
IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Centaurin alpha 1 antibody (ab27476)
This product has been referenced in:
- Moore CDet al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P; Human, Rat.Read more (PubMed: 17635995) »
See 1 publication for this product
Publishing research using ab27476? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"