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Western blot - Centaurin alpha 1 antibody (ab27476)
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Product Name
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Centaurin alpha 1 antibody
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See all Centaurin alpha 1 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Centaurin alpha 1
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Immunogen
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Synthetic peptide: CQEYAVEAHFKHKP, corresponding to amino acids 362-374 of Human Centaurin alpha 1.
BLAST 'CQEYAVEAHFKHKP' with  or 
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Reacts with
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/32000.
ICC/IF: Use at an assay dependent dilution PMID 17635995.
IHC-P: Use at an assay dependent dilution PMID 17635995.
WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human and mouse brain lysates for Western blot.
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Cellular localization
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Cytoplasmic, Nuclear and Plasma membrane
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Research areas
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Signal Transduction >> Signaling Pathway >> Lipid Signaling >> Other
Signal Transduction >> Adapters >> Cytoplasmic
Neuroscience >> Neurology process >> Neural Signal Transduction
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Relevance
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The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.
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Centaurin alpha 1 antibody (ab27476) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3. Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Centaurin alpha 1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Centaurin alpha 1 antibody images, references, products related to ab27476 and other tools.
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Centaurin alpha 1 antibody images:
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 Western blot - Centaurin alpha 1 antibody (ab27476)
Centaurin alpha 1 antibody (ab27476) at 1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size : 43 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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References for Centaurin alpha 1 antibody (ab27476)
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This product has been used in:
Moore CD et al. The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation. J Cell Sci 120:2683-93 (2007). WB, ICC/IF, IHC-P; Human, Rat. PubMed: 17635995
If you publish research using ab27476 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Centaurin alpha 1
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Centaurin alpha 1 antibody - more information
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