Ceruloplasmin Human ELISA Kit (ab108817)
- Product nameCeruloplasmin Human ELISA KitSee all Ceruloplasmin kits ...
- Detection methodColorimetric
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 0.6 µg/ml
- Range0.625 µg/ml - 40 µg/ml
- Assay time3h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Ceruloplasmin is an abundant alpha 2 serum glycoprotein that contains 95% of the copper found in the plasma of vertebrate species. Ceruloplasmin is a copper-binding protein that normally removes iron from cells by its ferroxidase activity. Ceruloplasmin concentration on average is 14.6 (±4.0) mg/dl. Low levels of ceruloplasmin lead to the abnormal deposition of iron in cells, including those of the pancreas, liver, retina and the basal ganglia region of the brain. Some diseases associated with ceruloplasmin are Wilson's disease, Hemochromatosis, Menkes disease and Aceroluplasminemia.
ab108817 is designed for detection of human ceruloplasmin in plasma and serum. This assay employs a quantitative competitive enzyme immunoassay technique that measures human ceruloplasmin in less than 3 hours. A polyclonal antibody specific for human ceruloplasmin has been pre-coated onto a 96-well microplate with removable strips. Ceruloplasmin in standards and samples is competed by a biotinylated ceruloplasmin sandwiched by the immobilized antibody and streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Biotinylated Ceruloplasmin 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Human Ceruloplasmin Microplate 1 x 96 tests Human Ceruloplasmin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl Wash Buffer Concentrate (20x) 1 x 30ml
- Research Areas
- CPCP 2Ferroxidase
- SwissProt: P00450 Human
Our Abpromise guarantee covers the use of ab108817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Ceruloplasmin Human ELISA Kit (ab108817)
ab108817 has not yet been referenced specifically in any publications.