Overview

  • Product nameCeruloplasmin Human ELISA Kit
    See all Ceruloplasmin kits
  • Detection methodColorimetric
  • Precision
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 1 ng/ml
  • Range
    1.25 ng/ml - 320 ng/ml
  • Recovery

    99 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Ceruloplasmin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin concentrations in urine, saliva, milk and culture supernatants.

    A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Ceruloplasmin Human ELISA Kit images

  • Representative Standard Curve using ab108818

Protocols

References for Ceruloplasmin Human ELISA Kit (ab108818)

ab108818 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone call this morning.

I have now received the confirmation from the laboratory that in the standard vial you received is indeed an amount of 320ng.

I apologize again for this missing information.

Plea...

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Thank you for contacting us. The standard is 320 ng to be mixed with 1 ml diluent to create the 320 ng/ml solution.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your patience while I contacted the lab. They have informed my that ab108818, would not be suitable for serum samples and that it only has around 20% cross-reactivity with monkey samples. However, ab108817, will react with...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"