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Abcam’s Ceruloplasmin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin concentrations in urine, saliva, milk and culture supernatants.
A Ceruloplasmin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Ceruloplasmin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Ceruloplasmin captured in plate.
|Components||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human Ceruloplasmin Antibody||1 x 140µl|
|Ceruloplasmin Microplate (12 x 8 well strips)||1 unit|
|Ceruloplasmin Standard||1 vial|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
CERP measured in culture supernatants (duplicates +/- SD). Samples were diluted in the range of 1/1-1/30.
CERP measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested after dilution 3000-30000 times, milk diluted 30-300 times, and other samples 1-10 times.
Representative Standard Curve using ab108818
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"