Ceruloplasmin Rat ELISA kit (ab108820)
Overview
- Product nameCeruloplasmin Rat ELISA kitSee all Ceruloplasmin kits ...
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeSandwich
- Sensitivity= 0.7 µg/ml
- Range0.78 µg/ml - 50 µg/ml
- Recovery
95 %
- Assay time3h 00m
- Species reactivityReacts with: Rat
- Product overview
Ceruloplasmin is an abundant a2-serum glycoprotein that contains 95% of the copper found in the plasma of vertebrate species. Ceruloplasmin is a copper-binding protein that normally removes iron from cells by its ferroxidase activity. Ceruloplasmin concentration on average is 14.6 (±4.0) mg/dl (2). Low levels of Ceruloplasmin lead to the abnormal deposition of iron in cells, including those of the pancreas, liver, retina and the basal ganglia region of the brain. Some diseases associated with Ceruloplasmin are Wilson's disease, Hemochromatosis, Menkes disease and Aceroluplasminemia.
ab108820 is designed for the detection of Human albumin in Plasma and Serum. This assay employs a quantitative sandwich enzyme immunoassay technique that measures Rat Ceruloplasmin in less than 3 hours. A polyclonal antibody specific for Rat Ceruloplasmin has been pre-coated onto a 96-well microplate with removable strips. Ceruloplasmin in standards and samples is sandwiched by the immobilized polyclonal antibody and biotinylated polyclonal antibody specific for Rat Ceruloplasmin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The colour development is stopped and the intensity of the colour is measured.
- Tested applicationsELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Biotinylated Ceruloplasmin 1 unit Chromogen Substrate 1 x 8ml Diluent Concentrate (10x) 1 x 30ml Rat Ceruloplasmin Microplate 1 x 96 units Rat Ceruloplasmin Standard 1 unit Stop Solution 1 x 12ml Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 90µl Wash Buffer Concentrate (20x) 1 x 30ml - Research Areas
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Alternative names
- CPCP 2Ferroxidase
Applications
Our Abpromise guarantee covers the use of ab108820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA |
Ceruloplasmin Rat ELISA kit images
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ELISA - Rat Ceruloplasmin ELISA kit 1 x 96 well plate (ab108820)Standard curve
The curve provided is for illustration only. A standard curve should be generated each time the assay is performed.
Protocols
References for Ceruloplasmin Rat ELISA kit (ab108820)
ab108820 has not yet been referenced specifically in any publications.
