Overview
- Product nameCervix (Human) Tissue Lysate - Squamous Cell CarcinomaSee all Cervix lysates ...
- General notesTissue: Cervix uteri Location: Cervix. Diagnosis: Squamous cell cervical carcinoma, moderately differentiated. Stage: I T1bNxM0 Grade: II Sex: Female Age: 54 years Size: 2.0 x 2.5 cm. Color: Pink Consistency: Firm Cut surface: White-gray Characteristics: Invaded, haemorragic, presence of calcifications Histologic pattern: Lymphocytic infiltration, Vascular invasion, Alveolar formation. Fibrosis, cystic degeneration and bleedin. No necrosis. Cellular differentiation: squamoid, spindle, pearl Insoluble protein fraction.
Properties
- FormLiquid
- Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
Constituents: PBS, 0.77% DTT, 0.1% SDS, 30.03% Urea, 15.22% Thiourea, 100% Laemmli buffer
Note: 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate -
Concentration information loading... - Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
- Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43884: Extraction 1: soluble protein fraction ab43883 Human Cervix Tumor Tissue lysate ab43882 Human Cervix Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43884 Human Cervix Tumor Tissue lysate
- Research Areas
References for Cervix (Human) Tissue Lysate - Squamous Cell Carcinoma (ab43884)
ab43884 has not yet been referenced specifically in any publications.
