Cervix (Human) Tissue Lysate - Squamous cell cervical carcinoma (ab43907)
- Product nameCervix (Human) Tissue Lysate - Squamous cell cervical carcinomaSee all Cervix lysates ...
- General notesTissue: Cervix uteri Location: Cervix Diagnosis: Squamous cell cervical carcinoma, moderately differentiated Stage: I T1b2NXM0 Grade: II Sex: Female Age: 31 years Appearance: Macroscopic: Organ: Cervix Size: 5 cm Color: Pink Consistency: Firm Cut surface: Gray-white Characteristics: +/- Encapsulated: - Invaded: + Hemorrhagic: + Cystic degeneration: + Calcification: - Histologic pattern: Cell distribution: +/- Diffuse: + Mosaic: - Necrosis: + Lymphocytic infiltration: + Vascular invasion: + Clusterized: - Alveolar formation: + Indian file: - Structure/Pattern: +/- Streaming: - Storiform: - Fibrosis: + Pallisading: + Cystic degeneration: - Bleeding: + Myxoid change: - Psammoma body: - Cellular differentiation: Squamous: +/- Squamoid: + Spindle: - Keratin: + Desmosome: - Pearl : - Adenomatous: +/- Glandular cell: - Cell stratification: - Secretion: - Intracellular vacuole: - Glandular formation: - Sarcomatous: +/- Round cell: - Spindle cell: - Leiomyoblast: - Lipoblast: - Rhadomyoblast: - Nuclear atypia: Nuclear appearance: 0 I II III Anisonucleosis: II Hyperchromatism: II Nucleolar prominent: II Multinucleated giant cell: II Mitotic activity: II Nuclear grade: II Insoluble protein fraction.
- Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferConstituents: 0.1% SDS, PBS, 5M Urea, 2M Thiourea, 50mM DTT, pH 7.4
Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
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- Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
- Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43907: Extraction 1: soluble protein fraction ab43905 Human Cervix Tumor Tissue lysate ab43904 Human Cervix Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43907 Human Cervix Tumor Tissue lysate ab43906 Human Cervix Normal Tissue lysate (matched)
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References for Cervix (Human) Tissue Lysate - Squamous cell cervical carcinoma (ab43907)
ab43907 has not yet been referenced specifically in any publications.