Cervix (Human) Tissue Lysate - adult normal tissue (ab43876)

Overview

  • Product nameCervix (Human) Tissue Lysate - adult normal tissue
    See all Cervix lysates
  • General notesDiagnosis: Normal Sex: Female Age: 21 Soluble protein fraction.

Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferConstituents: 0.25% Sodium Deoxycholate, 0.1% SDS, PBS, 1µg/ml Pepstatin A, 1µg/ml Leupeptin, 1µg/ml Aprotinin, 1mM PMSF, 1mM EDTA, 1mM Sodium fluoride, 1mM Sodium Orthovanadate, pH 7.4

    Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
  • Concentration information loading...
  • Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab43876: Extraction 1: soluble protein fraction ab43877 Human Cervix Tumor Tissue lysate ab43876 Human Cervix Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab43879 Human Cervix Tumor Tissue lysate ab43878 Human Cervix Normal Tissue lysate (matched)
  • Research Areas

    References for Cervix (Human) Tissue Lysate - adult normal tissue (ab43876)

    ab43876 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for patience. After looking into this further, it seems that we currently sell two fractions from each donor - the soluble and insoluble protein fractions. We do have many different donors available, but I am not sure if you need the soluble ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"