Overview
- Product nameAnti-Cezanne antibodySee all Cezanne primary antibodies ...
- DescriptionMouse monoclonal to Cezanne
- Tested applicationsWB, ELISA, IHC-P, Sandwich ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Recombinant fragment of Human Cezanne (amino acids 759-859) with proprietary tag, 11kDa. (NP_064590.2).
- Positive controlHuman endometrium tissue.
Properties
- FormLiquid
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferpH: 7.20
Constituent: 99% PBS -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- IsotypeIgG2b
- Light chain typekappa
-
Research Areas
Applications
Our Abpromise guarantee covers the use of ab118387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 93 kDa. |
| ELISA | ELISA: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Sandwich ELISA | sELISA: Use at an assay dependent dilution. |
Target
- FunctionHas deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B.
- Tissue specificityWidely expressed. Abundant in kidney, heart and fetal liver. Expressed differentially among B-cells at distinct developmental stages. Higher expression seen in primary immature B-cells as compared to the mature cells.
- Sequence similaritiesBelongs to the peptidase C64 family.
Contains 1 A20-type zinc finger.
Contains 1 OTU domain. - Cellular localizationCytoplasm. Nucleus.
-
Database links
- Entrez Gene: 56957 Human
- Entrez Gene: 56957 Human
- Entrez Gene: 56957 Human
- Omim: 611748 Human
- SwissProt: Q6GQQ9 Human
- SwissProt: Q6GQQ9 Human
- SwissProt: Q6GQQ9 Human
- Unigene: 98322 Human
Target information above from: UniProt accession
Q6GQQ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Cellular zinc finger anti NF kappa B protein antibodyCellular zinc finger anti-NF-kappa-B protein antibodyCEZANNE antibody
- HGNC:16683 antibodyOTU domain containing protein 7B antibodyOTU domain-containing protein 7B antibodyOTU7B_HUMAN antibodyOTUD7B antibodyZA20D1 antibodyZinc finger A20 domain containing protein 1 antibodyZinc finger A20 domain-containing protein 1 antibodyZinc finger protein Cezanne antibody
see all
Anti-Cezanne antibody images
-
Western blot - Anti-Cezanne antibody (ab118387)Anti-Cezanne antibody (ab118387) at 5 µg/ml + Recombinant immunogen at 0.2 µg
Predicted band size : 93 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cezanne antibody (ab118387)ab118387, at 3 ug/ml, staining Cezanne in formalin-fixed, paraffin-embedded Human endometrium tissue by Immunohistochemistry. -
Sandwich ELISA - Anti-Cezanne antibody (ab118387)Detection limit for ab118387 is 0.3 ng/ml as a capture antibody.
References for Anti-Cezanne antibody (ab118387)
ab118387 has not yet been referenced specifically in any publications.
