Overview

  • Product name
    ChIP Kit Magnetic - One Step
    See all ChIP Kit kits
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammal
  • Product overview

    Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In mammalian cells, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.


    Abcam's ChIP Kit Magnetic - One Step (ab156907) is a complete set of optimized buffers and reagents to carry out a successful chromatin immunoprecipitation for the selective enrichment of a chromatin fraction containing specific DNA sequences from isolated chromatin, in order to investigate the interaction between proteins and DNA in a cell. For best result, we recommend the use of Chromatin Extraction Kit (ab117152) for sample preparation.


     

  • Notes

    Primers

    The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.

     

  • Tested applications
    Suitable for: ChIPmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 48 tests 96 tests
    10X Wash Buffer 1 x 10ml 1 x 20ml
    8-Well Strip Caps 1 x 12 units 1 x 24 units
    96-Well PCR Plate 1 x 2 units 1 x 2 units
    Anti-RNA Polymerase II (1 mg/mL) 1 x 8µl 1 x 16µl
    ChIP Buffer 1 x 6ml 1 x 12ml
    DNA Release Buffer 1 x 14ml 1 x 28ml
    GAPDH Primer - Forward (20 µM) 1 x 8µl 1 x 16µl
    GAPDH Primer - Reverse (20 µM) 1 x 8µl 1 x 16µl
    Magnetic Beads 1 x 220µl 1 x 450µl
    Non-Immune IgG (1 mg/mL) 1 x 10µl 1 x 20µl
    Proteinase K (10 mg/mL) 1 x 55µl 1 x 110µl
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab156907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.

The GAPDH primers provided with the kit are for the human sequence. If using the kit with a difference species, GAPDH primers for that species will need to be acquired.

Images

  • The data shows the analysis of enrichment of RNA polymerase II in GAPDH and MLH1 promoters by Abcam's ChIP Kit Magnetic - One Step (ab156907) with chromatin extract prepared from formaldehyde fixed colon cancer cells. Captured DNA was used for analyzing levels of RNA polymerase II enriched in the GAPDH and MLH1 promoters.

Protocols

References

This product has been referenced in:
  • Kim TH & Cho SG Melatonin-induced KiSS1 expression inhibits triple-negative breast cancer cell invasiveness. Oncol Lett 14:2511-2516 (2017). Read more (PubMed: 28781689) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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