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Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of gene that affect homeostasis, development, and adaptation.
Abcam's ChIP Kit - Plants (ab117137) offers an advantageous tool for identifying direct genome-wide associations between specific regulatory proteins and their target genes in plant cells within 6 hours. The kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing and southern blot, as well as DNA microarray.
This kit is designed for 24 or 48 ChIP reactions, not for 24 or 48 samples. The standard protocol of the kit allows for performing 8 reactions with one sample. For testing more samples, the amount of each sample should be reduced. The amount of each reagent used for chromatin preparation should be also proportionally reduced.
|Components||24 tests||48 tests|
|100X Protease Inhibitor Cocktail||1 x 25µl||1 x 50µl|
|5X Lysis Buffer I||1 x 12ml||1 x 24ml|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Antibody Buffer||1 x 15ml||1 x 30ml|
|Anti-H3K9me2 (1 mg/mL)||1 x 5µl||1 x 8µl|
|Binding Buffer||1 x 5ml||1 x 8ml|
|ChIP Dilution Buffer||1 x 2ml||1 x 6ml|
|DNA Release Buffer||1 x 2ml||2 x 2ml|
|Elution Buffer||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Lysis Buffer II||1 x 3ml||1 x 6ml|
|Lysis Buffer III||1 x 2ml||1 x 4ml|
|Lysis Buffer IV||1 x 1.5ml||1 x 5ml|
|Normal Mouse IgG (1 mg/mL)||1 x 10µl||1 x 10µl|
|Proteinase K (10 mg/mL)||1 x 25µl||1 x 50µl|
|Reverse Buffer||1 x 2ml||2 x 2ml|
|Wash Buffer||1 x 28ml||2 x 28ml|
Our Abpromise guarantee covers the use of ab117137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"