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ab46666 |
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ab500 has been referenced in 5 publications.
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Chromatin immunoprecipitation using the Abcam ChIP kit with the Histone H3 antibody (ab1791). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Chromatin immunoprecipitation using the Abcam ChIP kit and the Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci). Primers and probes are located in the first kb of the transcribed region.
Chromatin immunoprecipitation using the Abcam ChIP kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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