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Products:Epigenetics and Nuclear Signaling >> Assays & Kits >> ChIP Related Products
Overview
Product name
Specificity
The Protein A ChIP Kit is compatible with rabbit immunoglobulin, various mouse immunoglobulin subtypes, and immunoglobulins from many other species. See the table in the protocol for more details.
Notes
The products will work for at least 2 years following purchase.
Store Protease Inhibitors, Proteinase K and Positive control antibody at 4°C (short term) or -20°C (long term). Avoid freeze-thaw cycles.
Store all other components at 4°C (do not freeze Protein A beads or DNA purifying slurry).
Store Protease Inhibitors, Proteinase K and Positive control antibody at 4°C (short term) or -20°C (long term). Avoid freeze-thaw cycles.
Store all other components at 4°C (do not freeze Protein A beads or DNA purifying slurry).
Tested applications
ChIPmore details
Properties
Storage instructions
Store at +4°C for short term use (1 week). Some components must not be frozen. Some components require storage at -20°C for long-term use (>1 week). See product manual for details.
Storage buffer
Kit Constituents: Please see protocol.
| Components | 1 kit |
|---|---|
| 1.25M Glycine | 1 x 10ml |
| Buffer A | 1 x 10ml |
| Buffer B | 1 x 30ml |
| Buffer C | 1 x 30ml |
| Buffer D | 1 x 3ml |
| ChIP Buffer 5X | 1 x 84ml |
| DNA Purifying Slurry | 1 x 3ml |
| H3 antibody ab1791 | 1 x 25µg |
| PCR-Grade Water | 1 x 10.2ml |
| Pre-blocked Protein A Beads | 1 x 960µl |
| Protease Inhibitors | 1 tablet |
| Proteinase K | 1 x 30µl |
Research areas
Kits/ Lysates/ Other >> Kits >> Epigenetic kits >> ChIP Kits
Epigenetics and Nuclear Signaling >> Assays & Kits >> ChIP Related Products
Alternative names
- ChIP Kit
Applications
Show applications key
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 3 Images
ChIP
Application notes
ChIP
ChIP Kit images:
ChIP - ChIP Kit (ab500)
Chromatin immunoprecipitation using the Abcam ChIP kit with the Histone H3 antibody (ab1791). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
ChIP - ChIP Kit (ab500)
Chromatin immunoprecipitation using the Abcam ChIP kit and the Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci). Primers and probes are located in the first kb of the transcribed region.
ChIP - ChIP Kit (ab500)
Chromatin immunoprecipitation using the Abcam ChIP kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Protocols
References for ChIP Kit (ab500)
This product has been referenced in:
- Yin Let al. Role of TMPRSS2-ERG Gene Fusion in Negative Regulation of PSMA Expression. PLoS One 6:e21319 (2011).Read more (PubMed: 21731703) »
- Anna Rawluszko Aet al. Butyrate Induces Expression of 17ß-Hydroxysteroid Dehydrogenase Type 1 in HT29 and SW707 Colorectal Cancer Cells. DNA Cell Biol : (2011).Read more (PubMed: 21563966) »
See all 5 publications for this product
Publishing research using ab500? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"