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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Assays & Kits >> ChIP Related Products
The Protein A ChIP Kit is compatible with rabbit immunoglobulin, various mouse immunoglobulin subtypes, and immunoglobulins from many other species. See the table in the protocol for more details.
ChIPmore details
Store at +4°C for short term use (1 week). Some components must not be frozen. Some components require storage at -20°C for long-term use (>1 week). See product manual for details.
Kit Constituents: Please see protocol.
| Components | 1 kit |
|---|---|
| 1.25M Glycine | 1 x 10ml |
| Buffer A | 1 x 10ml |
| Buffer B | 1 x 30ml |
| Buffer C | 1 x 30ml |
| Buffer D | 1 x 3ml |
| ChIP Buffer 5X | 1 x 84ml |
| DNA Purifying Slurry | 1 x 3ml |
| H3 antibody ab1791 | 1 x 25µg |
| PCR-Grade Water | 1 x 10.2ml |
| Pre-blocked Protein A Beads | 1 x 960µl |
| Protease Inhibitors | 1 tablet |
| Proteinase K | 1 x 30µl |
Kits/ Lysates/ Other >> Kits >> Epigenetic kits >> ChIP Kits
Epigenetics and Nuclear Signaling >> Assays & Kits >> ChIP Related Products
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP
ChIP - ChIP Kit (ab500)

Chromatin immunoprecipitation using the Abcam ChIP kit with the Histone H3 antibody (ab1791). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
ChIP - ChIP Kit (ab500)

Chromatin immunoprecipitation using the Abcam ChIP kit and the Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci). Primers and probes are located in the first kb of the transcribed region.
ChIP - ChIP Kit (ab500)

Chromatin immunoprecipitation using the Abcam ChIP kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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Chromatin immunoprecipitation using the Abcam ChIP kit with the Histone H3 antibody (ab1791). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

Chromatin immunoprecipitation using the Abcam ChIP kit and the Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci). Primers and probes are located in the first kb of the transcribed region.

Chromatin immunoprecipitation using the Abcam ChIP kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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