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The Protein A ChIP Kit is compatible with rabbit immunoglobulin, various mouse immunoglobulin subtypes, and immunoglobulins from many other species. See the table in the protocol for more details.
|Buffer A||1 x 10ml|
|Buffer B||1 x 30ml|
|1.25M Glycine||1 x 10ml|
|Buffer C||1 x 30ml|
|Buffer D||1 x 3ml|
|ChIP Buffer 5X||1 x 84ml|
|DNA Purifying Slurry||1 x 3ml|
|ab1791 - H3 antibody||1 x 25µg|
|PCR-Grade Water||1 x 10.2ml|
|Protease Inhibitors||1 tablet|
|Protein A Beads Unblocked||1 x 960µl|
|Proteinase K||1 x 30µl|
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
Chromatin immunoprecipitation assay was performed to define the interaction of serum response factor (SRF) with the intragenic serum response element (SRE) regulatory motif in mouse using ab500 ChIP Kit