Overview
- Product nameChIP KitSee all ChIP Kit kits ...
- Product overview
The Protein A ChIP Kit is compatible with rabbit immunoglobulin, various mouse immunoglobulin subtypes, and immunoglobulins from many other species. See the table in the protocol for more details.
- Notes
The products will work for at least 2 years following purchase.
Store Protease Inhibitors, Proteinase K and Positive control antibody at 4°C (short term) or -20°C (long term). Avoid freeze-thaw cycles.
Store all other components at 4°C (do not freeze Protein A beads or DNA purifying slurry).
- Tested applicationsChIP more details
Properties
- Storage instructionsPlease refer to protocols.
- Storage bufferKit Constituents: Please see protocol.
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Components 1 kit 1.25M Glycine 1 x 10ml Buffer A 1 x 10ml Buffer B 1 x 30ml Buffer C 1 x 30ml Buffer D 1 x 3ml ChIP Buffer 5X 1 x 84ml DNA Purifying Slurry 1 x 3ml ab1791 - H3 antibody 1 x 25µg PCR-Grade Water 1 x 10.2ml Protease Inhibitors 1 tablet Protein A Beads Unblocked 1 x 960µl Proteinase K 1 x 30µl - Research Areas
Applications
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ChIP |
ChIP Kit images
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ChIP - ChIP Kit (ab500)Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of transcribed region. -
ChIP - ChIP Kit (ab500)Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci). Primers and probes are located in the first kb of the transcribed region. -
ChIP - ChIP Kit (ab500)Chromatin immunoprecipitation using ab500 ChIP Kit and Histone H3 antibody (ab1791). Chromatin was prepared from HeLa cells using the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). Immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active/inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
Protocols
References for ChIP Kit (ab500)
This product has been referenced in:
- Espéli M et al. Analysis of a wild mouse promoter variant reveals a novel role for Fc?RIIb in the control of the germinal center and autoimmunity. J Exp Med 209:2307-19 (2012). Read more (PubMed: 23109709) »
- Mizuno T et al. YAP induces malignant mesothelioma cell proliferation by upregulating transcription of cell cycle-promoting genes. Oncogene 31:5117-22 (2012). Read more (PubMed: 22286761) »
