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Product Name
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ChIP Kit
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Product type
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Kits
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Description
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ChIP Kit
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Specificity
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The Protein A ChIP Kit is compatible with rabbit immunoglobulin, various mouse immunoglobulin subtypes, and immunoglobulins from many other species. See the table in the protocol for more details.
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Research areas
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Chromatin and Nuclear Signaling >> ChIP Related Products >> ChIP Related Products
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Components
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Kit Components | Unit Size/Volume |
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Pre-blocked Protein A Beads | 960 µl | H3 Antibody ab1791 | 25 µg | Buffer A | 10 ml | 1.25M Glycine | 10 ml | Buffer B | 30 ml | Buffer C | 30 ml | Buffer D | 3 ml | ChIP Buffer 5X | 84 ml | Protease Inhibitors | 1 tablet | DNA Purifying Slurry | 3 ml | PCR-Grade Water | 10.2 ml |
Proteinase K | 30 µl |
Note: Please click here for Instructions for use / protocol
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Storage instructions
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Store at +4°C for short term use (1 week). Some components must not be frozen. Some components require storage at -20°C for long-term use (>1 week). See product manual for details.
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Storage buffer
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Kit Constituents: Please see protocol.
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Tested applications
(see key)
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ChIP
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Application notes
(see key)
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Recommended dilutions
ChIP
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Notes
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The products will work for at least 2 years following purchase.
Store Protease Inhibitors, Proteinase K and Positive control antibody at 4°C (short term) or -20°C (long term). Avoid freeze-thaw cycles.
Store all other components at 4°C (do not freeze Protein A beads or DNA purifying slurry).
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Relevance
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Chromatin Immunoprecipitation kits (ChIP kits) enable the mapping of proteins and histone modifications to specific loci in the genome. This kit provides a positive control antibody together with protein A beads enabling the immunoprecipitation of chromatin/DNA complexes. The DNA region of interest can be quantified using quantitative PCR.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ChIP Kit is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ChIP Kit images, references, products related to ab500 and other tools.
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ChIP Kit images:
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 ChIP - ChIP Kit (ab500)
Chromatin immunoprecipitation using the Abcam ChIP kit with the Histone H3 antibody (ab1791). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab1791 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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ChIP - ChIP Kit (ab500)
Chromatin immunoprecipitation using the Abcam ChIP kit and the Histone H3 (tri methyl K4) antibody (ab12209). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 5 µg of ab12209 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci). Primers and probes are located in the first kb of the transcribed region.
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ChIP - ChIP Kit (ab500)
Chromatin immunoprecipitation using the Abcam ChIP kit and Histone H3 (tri methyl K9) antibody (ab8898). Chromatin was prepared from Hela cells according to the Abcam ChIP kit protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 2 µg of ab8898 (blue). No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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References for ChIP Kit (ab500)
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This product has been used in:
Cervera AM et al. Inhibition of succinate dehydrogenase dysregulates histone modification in mammalian cells. Mol Cancer 8:89 (2009). PubMed: 19849834
If you publish research using ab500 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to ChIP Kit
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ChIP Kit - more information
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Customer reviews (feedback) regarding ChIP Kit |
Customer FAQs regarding ChIP Kit |
Protocols for ChIP Kit |
Price and availability of products related to ChIP Kit |
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ChIP Kit - related products:
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ChIP Kit - other tools:
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Contact Abcam with a Technical Enquiry about ChIP Kit |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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