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The Protein A ChIP Kit is compatible with rabbit immunoglobulin, various mouse immunoglobulin subtypes, and immunoglobulins from many other species. See the table in the protocol for more details.
|Buffer A||1 x 10ml|
|Buffer B||1 x 30ml|
|1.25M Glycine||1 x 10ml|
|Buffer C||1 x 30ml|
|Buffer D||1 x 3ml|
|ChIP Buffer 5X||1 x 84ml|
|DNA Purifying Slurry||1 x 3ml|
|ab1791 - H3 antibody||1 x 25µg|
|PCR-Grade Water||1 x 10.2ml|
|Protease Inhibitors||1 tablet|
|Protein A Beads Unblocked||1 x 960µl|
|Proteinase K||1 x 30µl|
Our Abpromise guarantee covers the use of ab500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
Chromatin immunoprecipitation assay was performed to define the interaction of serum response factor (SRF) with the intragenic serum response element (SRE) regulatory motif in mouse using ab500 ChIP Kit
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"