Overview
Product nameChagas TcF protein
Protein descriptionRecombinant Trypanosoma cruzi, Chagas TcF Antigen.
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesab73161 is purified from this recombinant by using Histidine Nickel chromatography. Protein is >90% pure as determined by 10% PAGE (coomassie staining).
Biological activityImmunoreactive with sera patients infected with T. cruzi
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 0.4M Sodium chloride, 20mM Borate buffered saline, pH 7.8-8.2
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab73161
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
|
| ELISA |
|
Application notesELISA: a 1/1000 dilution of a 0.5 mg/ml solution coats at 50 ng/well.
WB: Use at an assay dependent dilution. Predicted molecular weight: 14.2 kDa.
ab73161 is an excellent antigen for detection of patients infected with T. cruzi with minimal specificity problems.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
RelevanceTrypanosoma cruzi, better known as T. cruzi, is a deadly parasite that causes Chagas’ disease.
TcF is an artificially engineered recombinant antigen which contains tandem sequences of different T. cruzi-specific peptides. It can be used to detect specific antibodies in nearly all patients infected with T. cruzi, and avoids many of the problems of false positivity and unreliability associated with older assays that used whole organisms for antibody detection.
References for Chagas TcF protein (ab73161)
ab73161
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
