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Anti-Chd7 antibody (ab31824)

This fast track antibody is not yet fully characterised. It is subject to these terms and conditions

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Buying a fast track?

We are able to bring you this product at a low price as it is not yet fully characterized. Our Scientific Support team are available to assist you, but we cannot offer refunds or replacements on this product.

What is a fast track? »

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1 question for ab31824

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Question 1

Monday 19-December-2011

LOT NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM We are planning to use this antibody for ChIP as it has been published to work in this application (mainly papers by Schnetz et al.). I seem to remember that the first batch we bought from you was labelled "ChiP-grade" but the most recent batches are not. Could you please explain what this means i.e. has the preparation method changed or have the recent batches failed on q control for ChIP applications? Thanks, Albert SAMPLE Mouse tissue PRIMARY ANTIBODY ? DETECTION METHOD ? POSITIVE AND NEGATIVE CONTROLS USED Will have Chd7-deficient tissue as negative control ANTIBODY STORAGE CONDITIONS 4oC SAMPLE PREPARATION 10mil cells X-CHIP or N-CHIP X-chip CROSSLINKING ? DNA FRAGMENTATION ? IP STEP ? DECROSSLINKING ? DNA PURIFICATION ? PELLET AFTER PRECIPITATION ? PRIMERS TESTED ON GENOMIC/INPUT DNA ? HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 0 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

ANSWER:

  

Thank you for your inquiry and I apologize for the delay.

I heard back from our in-house Business Development team that this antibody is still considered "fast track," meaning that it is only guaranteed to work in ELISA against the immunizing peptide.

http://www.abcam.com/assets/popups/popup_inv_grade.htm

We downgraded this antibody from being guaranteed in ChIP to being fast track since we couldn't test it in-house in ChIP for lot variations.  We were looking for a collaborator and will bring this to the attention of our BD team so that they can refocus on this product.

Thank you for bringing this to our attention.  Please let me know if you have any other questions.

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