Human Chemerin ELISA Kit (ab155430)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.51 ng/ml - 50 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Chemerin ELISA Kit
See all Chemerin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall < 10% Inter-assay Sample n Mean SD CV% overall < 12% -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.5 ng/ml -
Range
0.51 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum = 126.3 107% - 145% Plasma = 134.6 105% - 144% Cell culture media = 136.9 120% - 146% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Chemerin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human Chemerin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Chemerin coated on a 96-well plate. Standards and samples are pipetted into the wells and Chemerin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Chemerin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chemerin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimisation may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human Chemerin 2 vials Chemerin Microplate (12 x 8 wells) 1 unit Recombinant Human Chemerin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
This gene encodes a secreted chemotactic protein that initiates chemotaxis via the ChemR23 G protein-coupled seven-transmembrane domain ligand. Expression of this gene is upregulated by the synthetic retinoid tazarotene and occurs in a wide variety of tissues. The active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein. -
Cellular localization
Secreted -
Alternative names
- chemerin
- HP10433
- RAR-responsive protein TIG2
see all -
Database links
- Entrez Gene: 5919 Human
- Omim: 601973 Human
- SwissProt: Q99969 Human
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab155430 has been referenced in 6 publications.
- Oldfield L et al. Blood levels of adiponectin and IL-1Ra distinguish type 3c from type 2 diabetes: Implications for earlier pancreatic cancer detection in new-onset diabetes. EBioMedicine 75:103802 (2022). PubMed: 34990893
- Mir MM et al. Differential Association of Selected Adipocytokines, Adiponectin, Leptin, Resistin, Visfatin and Chemerin, with the Pathogenesis and Progression of Type 2 Diabetes Mellitus (T2DM) in the Asir Region of Saudi Arabia: A Case Control Study. J Pers Med 12:N/A (2022). PubMed: 35629157
- Tretjakovs P et al. Thioredoxin-1 and Correlations of the Plasma Cytokines Regarding Aortic Valve Stenosis Severity. Biomedicines 9:N/A (2021). PubMed: 34440245
- Song F et al. Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients. Front Endocrinol (Lausanne) 11:177 (2020). PubMed: 32300333
- Léniz A et al. Chemerin concentrations in infants born small for gestational age: correlations with triglycerides and parameters related to glucose homeostasis. J Physiol Biochem N/A:N/A (2020). PubMed: 32557228
- Fontes VS et al. Analysis of the chemerin and resistin adipokines in children and adolescents. Rev Assoc Med Bras (1992) 66:300-306 (2020). PubMed: 32520149