Anti-Chemerin antibody (ab72965)

Overview

• Product nameAnti-Chemerin antibodySee all Chemerin primary antibodies ...
• Description
  Mouse polyclonal to Chemerin
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length Human Chemerin protein (NP_002880.1, 1 a.a. ~ 163 a.a).

• Positive controlChemerin transfected 293T cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Hydrolysis

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Blood
  • Other

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Hydrolysis

  • Cardiovascular
  • Lipids / Lipoproteins
  • Adipose Related
  • Acrp

Applications

Target

• Tissue specificityHighly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon.
• Cellular localizationSecreted.

Target information above from: UniProt accession Q99969 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5919 Human
  • Omim: 601973 Human
  • SwissProt: Q99969 Human
  • Unigene: 647064 Human

• Alternative names
  HP10433 antibodyRAR responsive protein TIG2 antibodyRAR-responsive protein TIG2 antibody

  RARR2_HUMAN antibodyRARRES2 antibodyRetinoic acid receptor responder (tazarotene induced) 2 antibodyRetinoic acid receptor responder protein 2 antibodyTazarotene induced gene 2 protein antibodyTazarotene-induced gene 2 protein antibodyTIG2 antibody

  see all

Anti-Chemerin antibody images

• 

  Western blot - Chemerin antibody (ab72965)
  All lanes : Anti-Chemerin antibody (ab72965) at 1/500 dilution
  
  Lane 1 : Chemerin transfected 293T cell lysate
  Lane 2 : Non-transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 19 kDa
  Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Chemerin antibody (ab72965)
  ICC/IF image of ab72965 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.