Overview

  • Product nameAnti-Chemerin antibodySee all Chemerin primary antibodies ...
  • Description
    Mouse polyclonal to Chemerin
  • Tested applicationsICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Chemerin protein (NP_002880.1, 1 a.a. ~ 163 a.a).

  • Positive control
    • Chemerin transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab72965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Tissue specificityHighly expressed in skin (basal and suprabasal layers of the epidermis, hair follicles and endothelial cells). Also found in pancreas, liver, spleen, prostate, ovary, small intestine and colon.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • HP10433 antibody
    • RAR responsive protein TIG2 antibody
    • RAR-responsive protein TIG2 antibody
    • RARR2_HUMAN antibody
    • RARRES2 antibody
    • Retinoic acid receptor responder (tazarotene induced) 2 antibody
    • Retinoic acid receptor responder protein 2 antibody
    • Tazarotene induced gene 2 protein antibody
    • Tazarotene-induced gene 2 protein antibody
    • TIG2 antibody
    see all

Anti-Chemerin antibody images

  • All lanes : Anti-Chemerin antibody (ab72965) at 1/500 dilution

    Lane 1 : Chemerin transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 19 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab72965 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72965, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Chemerin antibody (ab72965)

This product has been referenced in:
  • Sell H  et al. Chemerin is a novel adipocyte-derived factor inducing insulin resistance in primary human skeletal muscle cells. Diabetes 58:2731-40 (2009). WB ; Human . Read more (PubMed: 19720798) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1x Citrate buffer
Sample Human Tissue sections (Adrenal tissue)
Specification Adrenal tissue
Permeabilization No
Fixative Formaldehyde
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Submitted Aug 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"