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Chicken-Erythrocyte Histone Octamers protein (Loading Control) (ab45275)

Submit an Abreview Q&A (8)

Overview

  • Product nameChicken-Erythrocyte Histone Octamers protein (Loading Control)

    • Properties

  • FormLiquid
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 2M Potassium chloride, 200mM Monobasic dihydrogen potassium phosphate, 200mM Dibasic monohydrogen potassium phosphate, 2.5mM Benzamidine Hydrochloride
  • Concentration information loading...
  • Additional notesThe histone octamers should be thawed very slowly in a cold room to avoid precipitation of KCl / phosphate. For stability ab45275 should be kept below 0°.

    ab45275 contains Benzamidine Hydrochloride and a MSDS can be supplied upon request.
  • Research Areas

    • Applications

  • Application notesab45275 can be used as a loading control for detecting post-translational modifications of H3, H2B, H2A and H4 as well as being used in a variety of enzymatic assays.

    • Protein info

    • Alternative names
        chromatin lysatechromatin preparationchromatin preparation
        histonehistone octamers
      see all
  • Relevanceab45275 provides 5mg of pure chicken erythrocyte native histone octamers. The octamers have been crystallized and the structure published to 1.90å resolution. The requirement for high yields of ultra pure proteins for X-ray crystallography studies and mass-spectrometry studies has stimulated a development programme to purify proteins from the nuclei of chicken erythrocytes under very gentle conditions. Thus native histone octamers can be produced without denaturation, with all the naturally post-translational modifications.

    • Chicken-Erythrocyte Histone Octamers protein (Loading Control) images

    • SDS-PAGE - Chicken-Erythrocyte Histone Octamers protein (Loading Control) (ab45275)
      SDS-PAGE - Chicken-Erythrocyte Histone Octamers protein (Loading Control) (ab45275)
      ab45275 separated using SDS-PAGE under non-reducing (lane 1) and reducing (lane 2) conditions. ab45275 contains the components of the histone octamer H3, H2B, H2A and H4.

    References for Chicken-Erythrocyte Histone Octamers protein (Loading Control) (ab45275)

    ab45275 has not yet been referenced specifically in any publications.

    Publishing research using ab45275 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab45275 Submit a Question for ab45275

    Displaying 1 - 8 of 8 results for Abreviews and Q&A

    10
    Votes: Highest First

    Thanks for the answer.
    However my question is "when" do the octamers become unstable when stored at -80degC and stored in the supplied buffer.
    My stock is about 2 years old. I want to know if they are unstable.
    thanks

    Thank you for contacting us.

    The octamers are expected to be stable for a year, I am sorry we are unable to tell you how far they will be stable e.g. after 18 or 24 months - we unfortunaltey haven't done any stability test for this product.
    <...

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    Customer have been using protein successfully since 2 years, however the current aliquots started giving weird results seems like octomer dissociating itself. They would like to know the shelf life of the product?

    Thank you for contacting us.

    I have finally received an answer from laboratory. They have mentioned that If the octamers are stored at -80 degrees in 20 % glycerol as supplied, they should OK after even a year. However when the samples are used, ...

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    using ab45275 infunctional assay

    Thank you for your call.

    I can now confirm that ab45275 can be dialyzed with leaving the octamers intakt by using 2M KCl, 0.2M K2HPO4, 0.2M KH2PO4, 2.5 mM benzamiding hydrochloride to dialyze into.

    Histone core octamers only stay intac...

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    Hello I am trying to run BN nativePAGE to see the histone octamers, but I dont see anything in the gel ? I ran the native protein gel using Invitrogen's NativePAGE™ Novex® Bis-Tris Gel System using G-250 to impart net negative charge on the protein co...

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    If the octamers are kept at -80C in 2M KCl, 0.2M K2HPO4, 0.2M KH2PO4, 20% glycerol, they may be thawed on standing at 4C for an hour or two. If they are dialysed into the buffer without the glycerol, they will be fine. Dialysis into 2M KCl with no buffer...

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    I am trying to run BN nativePAGE to see the histone octamers, but I dont see anything in the gel ? I ran the native protein gel using Invitrogen's NativePAGE™ Novex® Bis-Tris Gel System using G-250 to impart net negative charge on the protein complex. ...

    Read More

    Histone core octamers are not intact except in high salt, in the case of the product that is 2M KCl, 0.2M K2HPO4, 0.2M KH2PO4. They break into histone dimers of H2A and H2B and histone tetramers of two copies of H3 and H4 if they are dialysed into 100mM ...

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    1. what is the Storage life time of these octamers and how long will they stay as octamers and not disintegrate into individual histones? 2. Can use these octamers to make control nucleosomes (by adding DNA)? 3. Storage buffer contains 20% glycerol...

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    Please see also answer below. Nucleosome core particles can be constructed from the octamers, but assembly is to put tetramers on the DNA first and then the dimers. It is best to use defined sequences for the DNA. A protocol is in the reference: Asymm...

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    Can you please confirm that this octamers are assembled? Can you please also let us know what ration of those proteins are in an octamer? Further, do you have any idea about the shell-live of this product at -80 and at +4? Thank you very much!

    The octamers are intact and should crystallise as octamers to high resolution. However they should be treated as in the data and not allowed to be reduced in ionic strength from 2M KCl. 0.4 M equimolar potassium phosphate. They should be pretty well sto...

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    Is this pure histone octamer protein, or nucleosomes (incl DNA)?

    Thank you for your enquiry. ab45275 is free from DNA; pure histone octamers. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"