General notesab45274 can be thawed in a cold room and dialysed into any chosen protein buffer without glycerol (unless refreezing is needed). If the Mg2Cl2 is not needed it should be removed before using buffers that could cause precipitation of magnesium salts.
ab45274 contains Benzamidine Hydrochloride and a MSDS can be supplied upon request.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Backgroundab45274 provides 3mg of chick-erythrocyte nuclear lysate. Over 500 proteins have been identified in a previous batch by mass spectrometry including HDAC1, HDAC2 and SWI/SNF chromatin remodelling proteins. In addition a highly-enriched HMG fraction could be extracted and HMG1 and HMG2/2A purified.
The requirement for high yields of ultra pure proteins for X-ray crystallography studies and mass-spectrometry studies has stimulated a development programme to purify proteins from the nuclei of chick erythrocytes under very gentle conditions. ab45274 is a native protein nuclear extract prepared without denaturation, with all the naturally occurring variants and post-translational modifications present.
Application notesab45274 contains >500 chicken nuclear proteins including HMGs, HDACs and Swi /Snf proteins with little or no histone contamination. ab45274 is appropriate for enzymatic or structural studies.
ab45274 protein extract was purified gently under native conditions from chicken-erythrocyte nuclei (proprietary method). Lane 2 of the above SDS-PAGE shows ab45274, derived from the nuclei of ab45273, demonstrating that the lysate has little or no histone contamination. Lane 1 shows proteins acid-extracted from chicken erythrocyte nuclei (see ab45273 for protocol).
References for Chicken-Erythrocyte Nuclear Lysate (ab45274)
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