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Chicken IgY polyclonal - Isotype control (ab37382)

Q&A (3)

Overview

  • Product nameChicken IgY polyclonal - Isotype controlSee all Chicken isotype controls ...
  • Tested applicationsFlow Cyt more details

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 100mM Borate buffered saline, pH 8.2
  • Concentration information loading...
  • PurityIgY fraction
  • Clonality Polyclonal
  • IsotypeIgY
  • Research Areas
    • Alternative names
        Chicken Isotype Control

    Applications

    Our Abpromise guarantee covers the use of ab37382 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Flow Cyt
  • Application notesFlow Cyt: Use <1µg for 106 cells.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Chicken IgY polyclonal - Isotype control (ab37382)

    ab37382 has not yet been referenced specifically in any publications.

    Publishing research using ab37382 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab37382

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    Abcam technical, I have ab63498 (chicken (IgY) polyclonal anti-Human Serum Albumin antibody (FITC)) and I am looking to purchase a suitable negative chicken IgY control. Could you suggest one from your category that preferably does not cross react with a ...

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    Thank you for contacting us. Unfortunately, I would not recommend using a chicken antibody against a different target (such as ab1394 or ab63504), as it cannot be guaranteed that they do not react with any human antigen. I would rather suggest to use a ch...

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    I tested the antibody on A549 as you suggested as a negative control (see below).  However, the cells stained very well unfortunately.  Any ideas as to why? I am now growing primary mouse kertinocyte cells to see if how they stain.  

    Thank you for your reply. A549 cells were recommended because they showed weak to no staining using a similar antibody.  Plus these are the results found in the Human Protein Atlas: http://www.proteinatlas.org/ENSG00000169252/cell/A-549 I'm not sure if I ...

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    Attached are pictures of either isotype control (ab37382) at 1:150 or 1:250 and beta-2-AR (ab13989) staining at 1:150 and 1:250.  The secondary used was rabbit polyclonal Dylight488 (ab 96955) at 1:500 for everything.  The serum for blocking is rabbit ser...

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    Thank you for your reply and for providing the requested information. After seeing the data and your protocol, I would like to make some suggestions that may improve your results. One of my colleagues who has worked with chicken antibodies in the past...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"