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Product Name
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Chicken IgY secondary antibody - H&L
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Product type
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Secondary antibodies
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Description
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Rabbit polyclonal to Chicken IgY - H&L (FITC)
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Immunogen
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chicken IgY whole molecule
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IM
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution (PMID 18983843).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
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Cellular localization
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Secreted
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Research areas
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Secondary antibodies >> anti-Chicken >> IgY >> Fluorophore >> FITC
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Relevance
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Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Purity
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IgG fraction
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Conjugation notes
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Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons)
Absorption Wavelength: 495 nm
Emission Wavelength: 528 nm
Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
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Storage buffer
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0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
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Purification notes
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Form
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Liquid
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Conjugation
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Fluorescein
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Concentration
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1.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Chicken IgY secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Chicken IgY secondary antibody - H&L (ab6749)
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This product has been used in: (two most recent references)
Kuo YC et al. Capillary electrophoresis of bone marrow stromal cells with uptake of heparin-functionalized poly(lactide-co-glycolide) nanoparticles during differentiation towards neurons. Electrophoresis 31:315-23 (2010). ICC/IF. PubMed: 20084630
Tomasi ML et al. S-adenosylmethionine regulates apurinic/apyrimidinic endonuclease 1 stability: implication in hepatocarcinogenesis. Gastroenterology 136:1025-36 (2009). ICC/IF. PubMed: 18983843
See all 3 publication references for this product.
If you publish research using ab6749 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to IgY
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