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Rabbit anti-Chicken IgY H&L (FITC) secondary antibody (ab6749)

Submit an Abreview Q&A (2)Specific References (3)

Overview

  • Product nameRabbit anti-Chicken IgY H&L (FITC) secondary antibodySee all IgY secondary antibodies ...
  • Description
    Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (FITC)
  • Target speciesChicken
  • Tested applicationsImmunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA more details
  • Immunogen

    chicken IgY whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Immunomicroscopy IM: Use at an assay dependent concentration.
Flow Cyt Flow Cyt: Use at an assay dependent dilution.
IHC-P IHC-P: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent concentration.
ICC/IF ICC/IF: 1/1000 - 1/5000. PubMed: 18983843
ELISA ELISA: 1/10000 - 1/50000.

References for Rabbit anti-Chicken IgY H&L (FITC) secondary antibody (ab6749)

This product has been referenced in:
  • Kuo YC  et al. Capillary electrophoresis of bone marrow stromal cells with uptake of heparin-functionalized poly(lactide-co-glycolide) nanoparticles during differentiation towards neurons. Electrophoresis 31:315-23 (2010). ICC/IF . Read more (PubMed: 20084630) »
  • Tomasi ML  et al. S-adenosylmethionine regulates apurinic/apyrimidinic endonuclease 1 stability: implication in hepatocarcinogenesis. Gastroenterology 136:1025-36 (2009). ICC/IF . Read more (PubMed: 18983843) »

See all 3 Publications for this product

Publishing research using ab6749 ? Please let us know so that we can cite the reference in this datasheet

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I want to label the antiserum of chicken, which product I should chose for my Flowcytomry analysis (specifically FITC) among ab6749,ab7114 and ab6873? I noticed that ab7114's immunogen is full length native chicken IgY (purified). In addition, whether the...

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Thank you for your enquiry. ab6749, ab7114 and ab6873 are all suitable for flow cytometry analysis, have all been raised against chicken IgY and are all FITC conjugated. There is very little difference between the three aside from the mass of antibody...

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What protocols have been used for this antibody in immunofluorescence? Blocking, incubation times, dilutions. Any specific reference?

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. We do not have a specific protocol for this antibody in immunofluorescence, or a specific reference. If you have any more questions, p...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"