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Rabbit anti-Chicken IgY H&L (TR) secondary antibody (ab6751)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product nameRabbit anti-Chicken IgY H&L (TR) secondary antibodySee all IgY secondary antibodies ...
  • Description
    Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (TR)
  • Target speciesChicken
  • Tested applicationsImmunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA more details
  • Immunogen

    chicken IgY whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesTexas RedTM Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 4.9 moles Texas Redä per mole of Rabbit IgG
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Immunomicroscopy IM: Use at an assay dependent concentration.
Flow Cyt Flow Cyt: Use at an assay dependent dilution.
IHC-P IHC-P: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent concentration.
ICC/IF ICC/IF: 1/1000 - 1/5000. (PMID 18482827).
ELISA ELISA: 1/10000 - 1/50000.

References for Rabbit anti-Chicken IgY H&L (TR) secondary antibody (ab6751)

This product has been referenced in:
  • Myöhänen TT  et al. Localization of prolyl oligopeptidase in the thalamic and cortical projection neurons: a retrograde neurotracing study in the rat brain. Neurosci Lett 450:201-5 (2009). IHC-FrFl . Read more (PubMed: 19041368) »
  • Crump D  et al. Detection of PBDE effects on mRNA expression in chicken (Gallus domesticus) neuronal cells using real-time RT-PCR and a new differential display method. Toxicol In Vitro 22:1337-43 (2008). ICC/IF . Read more (PubMed: 18482827) »

See all 2 Publications for this product

Publishing research using ab6751 ? Please let us know so that we can cite the reference in this datasheet

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Has this antibody been adsorbed against rat IgG?

Thank you for your recent technical service inquiry. We can not guarantee that this product will not cross react with rat as this has not been tested. In fact, they are close enough that it is possible that they will cross react. Product ab7116, Texas...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"