Chicken IgY secondary antibody - H&L (ab6752)

Product Name 

Chicken IgY secondary antibody - H&L

Product type 

Secondary antibodies

Description 

Rabbit polyclonal to Chicken IgY - H&L (Biotin)

Immunogen 

chicken IgY whole molecule

Tested applications 
(see key)

Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IM, WB

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent dilution (PMID 18423037).
IHC-P: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
IM: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Please refer to the relevant conjugation for dilutions specific for this product:

Cellular localization 

Secreted

Research areas 

Secondary antibodies >> anti-Chicken >> IgY >> Biotin

Relevance 

Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).

Raised in 

Rabbit

Clonality 

Polyclonal

Purity 

IgG fraction

Conjugation notes 

This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

Storage buffer 

0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA

Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS

Purification notes 

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

Form 

Liquid

Conjugation 

Biotin

Concentration 

1.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Chicken IgY secondary antibody - H&L (ab6752)

  This product has been used in: (two most recent references)

Koryakina YA et al. Characterization of a panel of six beta2-adrenergic receptor antibodies by indirect immunofluorescence microscopy. Respir Res 9:32 (2008). ICC/IF. PubMed: 18423037

Ergn S et al. Cell type-specific expression of LINE-1 open reading frames 1 and 2 in fetal and adult human tissues. J Biol Chem 279:27753-63 (2004). PubMed: 15056671
If you publish research using ab6752 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Chicken IgY secondary antibody - H&L

Customer FAQs regarding Chicken IgY secondary antibody - H&L

Protocols for Chicken IgY secondary antibody - H&L

Price and availability of products related to Chicken IgY secondary antibody - H&L