|
|
|
|
|
Product Name
|
Chicken IgY secondary antibody - H&L
|
|
Product type
|
Secondary antibodies
|
|
Description
|
Goat polyclonal to Chicken IgY - H&L (HRP)
|
|
Immunogen
|
chicken IgY whole molecule
|
|
Tested applications
(see key)
|
Dot, ELISA, IHC-Fr, IHC-P, IM, WB
|
|
Application notes
(see key)
|
Recommended dilutions
suitable for immunoblotting (dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
WB: Use at an assay dependent concentration (from PubMed:16543490).
Please refer to the relevant conjugation for dilutions specific for this product:
Conjugate |
ELISA |
Blotting |
Flow Cytometry |
IHC |
Fluorescein |
nd |
nd |
50 - 200 |
200 - 1,000 |
Rhodamine, Texas RedTM |
nd |
nd |
50 - 200 |
200 - 1,000 |
R-Phycoerythrin |
nd |
nd |
100 - 200 |
nd |
Cyanines |
nd |
nd |
400 |
100 - 1,000 |
Biotin |
5,000 - 20,000 |
3,000 - 10,000 |
nd |
100 - 1,000 |
Peroxidase |
4,000 - 10,000 |
2,000 - 5,000 |
nd |
200 - 2,000 |
Alkaline Phosphatase |
1,000 - 2,000 |
500 - 1,000 |
nd |
100 - 1,000 |
|
|
|
Cellular localization
|
Secreted
|
|
Research areas
|
Secondary antibodies >> anti-Chicken >> IgY >> Enzyme >> HRP
|
|
Relevance
|
Secreted immunoglobulins have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgY has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).
|
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Purity
|
IgG fraction
|
|
Conjugation notes
|
Horseradish Peroxidase (HRP)
|
|
Storage buffer
|
0.01M NaPhos, 0.14M NaCl, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Phosphate MSDS
|
|
Purification notes
|
This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
|
|
Form
|
Liquid
|
|
Conjugation
|
Horse Radish Peroxidase
|
|
Concentration
|
2.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Chicken IgY secondary antibody - H&L is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
References for Chicken IgY secondary antibody - H&L (ab6877)
|
|
This product has been used in: (two most recent references)
Wang X et al. Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice. J Cell Biol 184:143-58 (2009). WB. PubMed: 19139267
Kang Q & Chen A Curcumin suppresses expression of low-density lipoprotein (LDL) receptor, leading to the inhibition of LDL-induced activation of hepatic stellate cells. Br J Pharmacol 157:1354-67 (2009). PubMed: 19594758
See all 8 publication references for this product.
If you publish research using ab6877 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to IgY
|
| |
Chicken IgY secondary antibody - H&L - more information
|
Customer reviews (feedback) regarding Chicken IgY secondary antibody - H&L |
Customer FAQs regarding Chicken IgY secondary antibody - H&L |
Protocols for Chicken IgY secondary antibody - H&L |
Price and availability of products related to Chicken IgY secondary antibody - H&L |
| |
Chicken IgY secondary antibody - H&L - other tools:
|
Contact Abcam with a Technical Enquiry about Chicken IgY secondary antibody - H&L |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
