Chikungunya Virus IgM Human ELISA Kit (ab108717)
Overview
- Product nameChikungunya Virus IgM Human ELISA Kit
- Sample typeSerum, Plasma
- Assay typeSandwich
- Species reactivityReacts with: Human
- Product overview
ab108717, the Chikungunya IgM ELISA Kit is intended for the qualitative determination of IgM class antibodies to Chikungunya virus in Human serum and plasma (citrate).
Chikungunya virus is an arthropod borne virus of the genus Alphavirus (family Togaviridae). The Alphavirus genus contains at least 24 distinct species. These are lipid-enveloped virions with a diameter of 50 to 60 nm.
Alphavirus infections are initiated by the bite of an infected mosquito, which results in the deposition of virus in subcutaneous and possibly cutaneous tissues. After an incubation period of 1 to 12 days the Chikungunya fever develops. Chikungunya fever (Chikungunya means “that which bends up”, in reference to the crippling manifestations of the disease) is an acute viral infection characterized by a rapid transition from a state of good health to illness that includes severe arthralgia and fever. Temperature rises abruptly to as high as 40°C and is often accompanied by shaking chills. After a few days, fever may abate and recrudesce, giving rise to a “saddleback” fever curve. Arthralgia is polyarticular, favoring the small joints and sites of previous injuries, and is most intense on arising. Patients typically avoid movement as much as possible. Joints may swell without significant fluid accumulations. These symptoms may last from 1 week to several months and are accompanied by myalgia. The rash characteristically appears on the first day of illness, but onset may be delayed. It usually arises as a flush over the face and neck, which evolves to a maculopapular or macular form that may be pruritic. The latter lesions appear on the trunk, limbs, face, plams and soles, in that order of frequency. Petechial skin lesions have also been noted. Headache, photophobia, retro-orbitral pain, sore throat with objective signs of pharyngitis, nausea and vomiting also occur in this setting. Occasionally, however persistent arthralgia and polyarthritis (lasting months or even years) do occur, sometimes involving joint destruction. Even rarer, sequelae include encephalitis and meningoencephalitis with high lethality rates.
The virus has major importance in Africa and Asia. From 20% to more than 90% of the population of tropical and subtropical show serologic evidence of infection. Because Aedes mosquitoes are increasingly prevalent in North Africa and South America, where the population would be uniformly susceptible to infection, the possibility for epidemics is evident. Chikungunya virus infections are imported to central Europe mainly by travellers to tropical and subtropical countries.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 1 x 96 tests Chikungunya IgM Cut-off Control Yellow Bottle, Green Cap 1 x 2ml Chikungunya IgM Negative Control Yellow Bottle, Blue Cap 1 x 1.5ml Chikungunya IgM Positive Control Yellow Bottle, Red Cap 1 x 1.5ml Chikungunya Microplate (IgM) 1 unit Chikungunya Solution 1, lyophilised Red Cap 6 units Chikungunya Solution 2 Blue Bottle, White cap 1 x 6ml Cover foil 1 unit Sample Diluent Yellow Bottle, White Cap 1 x 100ml Stop Solution Red Cap 1 x 15ml Streptavidin conjugate Red Bottle, Black Cap 1 x 6ml Strip holder 1 unit TMB Substrate Solution Yellow Cap 1 x 15ml Washing Solution (20x conc.) White Cap 1 x 50ml - Research Areas
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Alternative names
- Chikungunya Virus IgM
Applications
Our Abpromise guarantee covers the use of ab108717 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Protocols
References for Chikungunya Virus IgM Human ELISA Kit (ab108717)
ab108717 has not yet been referenced specifically in any publications.
