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Anti-Chk1 antibody [E250] (ab32531)

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Reassurance, Refunds & Replacements

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Overview

Product name

Anti-Chk1 antibody [E250]
See all Chk1 products (30) ...

Description

Rabbit monoclonal [E250] to Chk1

Specificity

ab32531 recognises Chk1.

Tested applications

WB, ICC, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to N-terminal residues of human Chk1

Positive control

K562 cell lysate

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

E250

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Chk1 / Chk2

  • Western blot - Chk1 antibody [E250] (ab32531)Western blot - Chk1 antibody [E250] (ab32531) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/50.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Is unsuitable for FACS or IHC.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Target information above from: UniProt accessionO14757 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • C85740 antibody
  • Cell cycle checkpoint kinase antibody
  • Checkpoint , S. pombe, homolog of, 1 antibody
  • Checkpoint kinase 1 antibody
  • Checkpoint kinase 1 homolog (S. pombe) antibody
  • CHEK 1 antibody
  • CHEK1 antibody
  • Chk 1 antibody
  • Chk1 antibody
  • CHK1 checkpoint homolog (S. pombe) antibody
  • CHK1_HUMAN antibody
  • EC 2.7.11.1 antibody
  • rad27 antibody
  • Serine/threonine protein kinase Chk1 antibody
  • Serine/threonine-protein kinase CHK1 antibody
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
see all

Database links

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Anti-Chk1 antibody [E250] images:

  Western blot - Chk1 antibody [E250] (ab32531)

Western blot - Chk1 antibody [E250] (ab32531)

Anti-Chk1 antibody [E250] (ab32531) at 1/1000 dilution + K562 cell lysate

Predicted band size : 54 kDa
Observed band size : 54 kDa

References for Anti-Chk1 antibody [E250] (ab32531)

This product has been referenced in:

  • Luo MH  et al. Human cytomegalovirus disrupts both ataxia telangiectasia mutated protein (ATM)- and ATM-Rad3-related kinase-mediated DNA damage responses during lytic infection. J Virol 81:1934-50 (2007). WB; Human.Read more (PubMed: 17151099) »

See 1 publication for this product

Publishing research using ab32531? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"