![Western blot - Chk1 antibody [EP691Y] (ab40866) image](#ab40866wb.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Chk1 antibody [EP691Y] (ab40866) image](#ab40866IHCP.jpg)
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- Secondary antibodies
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Read our guarantee »Overview
Product name
Anti-Chk1 antibody [EP691Y]
See all Chk1 products (30) ...
Description
Rabbit monoclonal [EP691Y] to Chk1
Tested applications
ICC/IF, WB, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to C-terminal residues of human Chk1.
Positive control
HeLa cells lysate and human breast carcinoma.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP691Y
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Chk1 / Chk2
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/100 - 1/250.ICC/IF: 1/100 - 1/250.
WB: 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
Flow Cyt
Flow Cyt: 1/160.Flow Cyt: 1/160.
IHC-P: 1/250 - 1/500.
Target
Target information above from: UniProt accessionO14757
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
Database links
- Entrez Gene: 1111 Human
- Omim: 603078 Human
- SwissProt: O14757 Human
- Unigene: 24529 Human
Anti-Chk1 antibody [EP691Y] images:
Western blot - Chk1 antibody [EP691Y] (ab40866)
![Western blot - Chk1 antibody [EP691Y] (ab40866)](/ps/datasheet/Images/40/ab40866/ab40866wb.jpg)
Anti-Chk1 antibody [EP691Y] (ab40866) at 1/10000 dilution + HeLa cell lysate at 10 µg
Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunohistochemistry (Paraffin-embedded sections) - Chk1 antibody [EP691Y] (ab40866)
![Immunohistochemistry (Paraffin-embedded sections) - Chk1 antibody [EP691Y] (ab40866)](/ps/datasheet/Images/40/ab40866/ab40866IHCP.jpg)
Immunohistochemical analysis of Chk1 expression in paraffin-embedded human breast carcinoma using ab40866 at a 1:250 dilution.
References for Anti-Chk1 antibody [EP691Y] (ab40866)
This product has been referenced in:
- Leich E et al. MicroRNA profiles of t(14;18)-negative follicular lymphoma support a late germinal center B-cell phenotype. Blood 118:5550-8 (2011). IHC-P; Human.Read more (PubMed: 21960592) »
See 1 publication for this product
Publishing research using ab40866? Please let us know so that we can cite the reference in this datasheet
![Chk1 antibody [EP691Y] for Western blot in Human (40866)](/ps/datasheet/images/40/ab40866/Chk1-Primary-antibodies-ab40866-2.jpg)
![Chk1 antibody [EP691Y] for Western blot in Human (40866)](/ps/datasheet/images/40/ab40866/Chk1-Primary-antibodies-ab40866-7.jpg)
![Anti-Chk1 antibody [EP691Y] for Western blot in Human (40866)](/ps/datasheet/images/40/ab40866/Chk1-Primary-antibodies-ab40866-14.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"