Anti-Chk1 antibody (ab47574)

Overview

• Product nameAnti-Chk1 antibodySee all Chk1 primary antibodies ...
• Description
  Rabbit polyclonal to Chk1
• Tested applicationsICC/IF, WB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human, Fruit fly (Drosophila melanogaster)
  
• Immunogen

  Synthetic non phosphopeptide derived from human Chk1 around the phosphorylation site of serine 345 (S-F-S^P-Q-P).

• Positive controlHT29 cell extracts. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: MCF-7.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Kinases/phosphatases
  • Other

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • Chk1 / Chk2

Applications

Target

Target information above from: UniProt accession O14757 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1111 Human
  • Entrez Gene: 1111 Human
  • Entrez Gene: 1111 Human
  • Entrez Gene: 12649 Mouse
  • Entrez Gene: 140583 Rat
  • Omim: 603078 Human
  • SwissProt: O14757 Human
  • SwissProt: O14757 Human

  • SwissProt: O14757 Human
  • SwissProt: O35280 Mouse
  • SwissProt: Q91ZN7 Rat
  • Unigene: 24529 Human
  • Unigene: 16753 Mouse
  • Unigene: 33267 Rat

  see all

• Alternative names
  C85740 antibodyCell cycle checkpoint kinase antibodyCheckpoint , S. pombe, homolog of, 1 antibody

  Checkpoint kinase 1 antibodyCheckpoint kinase 1 homolog (S. pombe) antibodyCHEK 1 antibodyChek1 antibodyChk 1 antibodyChk1 antibodyCHK1 checkpoint homolog (S. pombe) antibodyCHK1_HUMAN antibodyEC 2.7.11.1 antibodyrad27 antibodySerine/threonine protein kinase Chk1 antibodySerine/threonine-protein kinase CHK1 antibodySTT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody

  see all

Anti-Chk1 antibody images

• 

  Western blot - Chk1 antibody (ab47574)
  All lanes : Anti-Chk1 antibody (ab47574) at 1/500 dilution
  
  Lane 1 : HT29 cell extract treated with UV. No peptide.
  Lane 2 : HT29 cell extract treated with UV. Synthetic peptide present.
  
  
  Predicted band size : 54 kDa
  Observed band size : 54 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Chk1 antibody(ab47574)
  ab47574 staining Chk1 in Human heart.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Chk1 antibody (ab47574)

  ICC/IF image of ab47574 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab47574 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.