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- Secondary antibodies
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Product name
Anti-Chk1 (phospho S317) antibody
See all Chk1 products (30) ...
Description
Rabbit polyclonal to Chk1 (phospho S317)
Specificity
Chk1(Phospho-Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317.
Tested applications
ICC/IF, ELISA, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 150% Sodium chloride, PBS (without Mg2+ and Ca2+), pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Chk1 / Chk2
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Western blot - Chk1 (phospho S317) antibody (ab38518)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ELISA
Application notes
ELISA: 1/4000.
ICC/IF: Use at an assay dependent dilution (PMID 19390600).
WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 54 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Target information above from: UniProt accessionO14757
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C85740 antibody
- Cell cycle checkpoint kinase antibody
- Checkpoint , S. pombe, homolog of, 1 antibody
see all
Database links
- Entrez Gene: 1111 Human
- Entrez Gene: 140583 Rat
- Omim: 603078 Human
- SwissProt: O14757 Human
- SwissProt: Q91ZN7 Rat
- Unigene: 24529 Human
- Unigene: 33267 Rat
Anti-Chk1 (phospho S317) antibody images:
Western blot - Chk1 (phospho S317) antibody (ab38518)
All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution
Lane 1 : Extracts from MCF7 cells (5-30ug of total protein)
Lane 2 : Extracts from COLO cells (5-30ug of total protein)
Secondary
Alkaline Phosphatase Goat Anti Rabbit IgG (H+L)
Predicted band size : 54 kDa
Observed band size : 63 kDa (why is the actual band size different from the predicted?)
References for Anti-Chk1 (phospho S317) antibody (ab38518)
This product has been referenced in:
- Kadaja M et al. Mechanism of genomic instability in cells infected with the high-risk human papillomaviruses. PLoS Pathog 5:e1000397 (2009). ICC/IF; Human.Read more (PubMed: 19390600) »
See 1 publication for this product
Publishing research using ab38518? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"