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Product name
Anti-Chk2 antibody
See all Chk2 products (27) ...
Description
Rabbit polyclonal to Chk2
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: SRESDVEAQQSHGSSAC, corresponding to amino acids 2-18 of Human Chk2.
SRESDVEAQQSHGSSAC
Positive control
Jurkat or K562 cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS with 0.02% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Chk1 / Chk2
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).Can be blocked with Chk2 (N-term) peptide (human) (ab6088).
ICC/IF: Use a concentration of 1 µg/ml.
Target
Function
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
Tissue specificity
High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
Involvement in disease
Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
Contains 1 FHA domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated by PLK4.
Cellular localization
Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
Target information above from: UniProt accessionO96017
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bA444G7 antibody
- CDS 1 antibody
- CDS1 antibody
see all
Database links
- Entrez Gene: 11200 Human
- Entrez Gene: 50883 Mouse
- Entrez Gene: 114212 Rat
- Omim: 604373 Human
- SwissProt: O96017 Human
- SwissProt: Q9Z265 Mouse
- SwissProt: Q9R019 Rat
- Unigene: 291363 Human
see all
Anti-Chk2 antibody images:
Western blot - Chk2 antibody (ab8108)
All lanes : Anti-Chk2 antibody (ab8108) at 1 µg/ml
Lane 1 : K562 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HL-60 whole cell lysate
Predicted band size : 61 kDa
Immunocytochemistry/ Immunofluorescence - Chk2 antibody (ab8108)
ab8108 at 1µg/ml staining Chk2 in Jurkat cells by ICC/IF
Immunocytochemistry/ Immunofluorescence - Chk2 antibody (ab8108)
ab8108 (1/100) staining Chk2 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
References for Anti-Chk2 antibody (ab8108)
This product has been referenced in:
- Brunton H et al. Analysis of human syndromes with disordered chromatin reveals the impact of heterochromatin on the efficacy of ATM-dependent G2/M checkpoint arrest. Mol Cell Biol 31:4022-35 (2011).Read more (PubMed: 21791604) »
- Sakasai R et al. Transcription-dependent activation of ataxia telangiectasia mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison. J Biol Chem 285:15201-8 (2010). WB; Human.Read more (PubMed: 20304914) »
See all 13 publications for this product
Publishing research using ab8108? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"