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Product name
Anti-Chk2 (phospho S516) antibody
See all Chk2 products (27) ...
Description
Rabbit polyclonal to Chk2 (phospho S516)
Specificity
anti-CHK2(Phospho-Ser516) antibody detects endogenous levels of CHK2 only when phosphorylated at Serine 516.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthesized phosphopeptide derived from human CHK2 around the phosphorylation site of Serine 516 (Q-P-SP-T-S).
Positive control
UV treated Hela Cell extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Chk1 / Chk2
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Western blot - Chk2 (phospho S516) antibody (ab36724)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
Target
Function
Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
Tissue specificity
High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
Involvement in disease
Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.
Defects in CHEK2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Defects in CHEK2 are found in some patients with osteogenic sarcoma (OSRC) [MIM:259500].
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.
Contains 1 FHA domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Phosphorylated by PLK4.
Cellular localization
Nucleus; Nucleus. Isoform 10 is present throughout the cell and Nucleus > PML body. Nucleus > nucleoplasm. Recruited into PML bodies together with TP53.
Target information above from: UniProt accessionO96017
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bA444G7 antibody
- bA444G7 antibody
- CDS 1 antibody
see all
Database links
- Entrez Gene: 11200 Human
- Omim: 604373 Human
- SwissProt: O96017 Human
- Unigene: 291363 Human
- Unigene: 505297 Human
Anti-Chk2 (phospho S516) antibody images:
Western blot - Chk2 (phospho S516) antibody (ab36724)
All lanes : Anti-Chk2 (phospho S516) antibody (ab36724) at 1/500 dilution
Lane 1 : Hela cell extract treated with UV
Lane 2 : Hela cell extract untreated
Lysates/proteins at 30 µg per lane.
Predicted band size : 61 kDa
Observed band size : 61 kDa
Lanes can be loaded with 5-30µg of total protein.
References for Anti-Chk2 (phospho S516) antibody (ab36724)
This product has been referenced in:
- Guo X et al. Interdependent phosphorylation within the kinase domain T-loop Regulates CHK2 activity. J Biol Chem 285:33348-57 (2010). WB; Human.Read more (PubMed: 20713355) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"