The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Direct competitive inhibition ELISA using immobilized anti-chloramphenicol (3ug/mL), and chloramphenicol-labelled HRP(100 ng/mL). 50% inhibition occurred at 50 ng/mL of free chloramphenicol in non-optimized conditions.
Use at an assay dependent dilution.
RelevanceChloramphenicol is an antibiotic that was derived from the bacterium Streptomyces venezuelae. It was the first antibiotic to be manufactured synthetically on a large scale. Chloramphenicol is effective against a wide variety of microorganisms, but due to serious side effects (eg damage to the bone marrow) in humans, it is usually reserved for the treatment of serious and life threatening infections (eg typhoid fever). It is also used in eye drops or ointment to treat bacterial conjunctivitis.
References for Anti-Chloramphenicol antibody (ab100978)
has not yet been referenced specifically in any publications.
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