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Cholesterol/Cholesteryl Ester Detection Kit (ab102515)

Q&A (6)

Overview

  • Product nameCholesterol/Cholesteryl Ester Detection KitSee all Cholesterol/Cholesteryl Ester kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeQuantitative
  • Assay time
    1h 00m
  • Product overview

    Abcam's Cholesterol/Cholesteryl Ester Detection Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions. In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in this kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceCholesterol is an essential molecule in all animal life. It is involved in both normal development and diseases. A high level of serum cholesterol is an indicator for diseases such as heart disease. Cholesteryl ester is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.

    • Applications

    Our Abpromise guarantee covers the use of ab102515 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Cholesterol/Cholesteryl Ester Detection Kit images

    • Functional Studies - Cholesterol/Cholesteryl Ester Detection Kit (ab102515)
      Functional Studies - Cholesterol/Cholesteryl Ester Detection Kit (ab102515)
      Cholesterol Standard Curve: Cholesterol/Cholesteryl Ester was quantified according to the kit instructions. Background from the 0 standard reading (without cholesterol) has been subtracted from all readings.

    Protocols

    References for Cholesterol/Cholesteryl Ester Detection Kit (ab102515)

    ab102515 has not yet been referenced specifically in any publications.

    Publishing research using ab102515 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab102515

    Displaying 1 - 6 of 6 results for Q&A

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    Votes: Highest First

    Dear
    Thank you for taking time to look at my question – will order a couple of kits and try it out.

    Kind regards

    I'm glad you found the information of help.

    I'd be very interested to know how you get on with the kit if you would mind letting me know. If you need any help with the kit please do not hesitate to contact us again.

    Until then, I wish ...

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    Phone call requesting information on it the Cholesterol/Cholesteryl Ester Quantitation Kit can be used with egg yolk samples.

    Thank you for contacting us yesterday.

    I now have some further information to share with you in regards to the Cholesterol/Cholesteryl Ester Quantitation Kit and Cholesterol/Cholesteryl Ester Detection Kit (ab65359 and ab102515 respectively). Read More

    I will be sending in an order today for abcam's cholesterols kit (ab102515 LDL/HDL/VLDL) urgently, but I have a question: For cholesterol determination in tissue samples it is required to extract lipids and after centrifugation air dry at 50 degrees and t...

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    Thank you for your inquiry.

    I can confirm that without the help of vacuum the drying process will be much longer.

    But it is possible to do so by prolonging the air dry period at 50C.

    Since this has not be experimentally teste...

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    Hello, We have order the Cholesterol/Cholesteryl Ester Kit, and were wondering if the kit is useble on mouse bile samples. Thanks!

    Thank you for contacting us.

    Yes, the kit is suitable for assay of mouse bile.

    Please do not hesitate to contact us if you need any more advice or information.

    Dear Abcam I would like to investigate the levels of Cholesterol and cholesterol esters in cell lysates or homogenated tissue. I see that you have two kits for this purpose. My question is: Is it possible to measure directly on cell lysate or does it requ...

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    Thank you for contacting us.

    Indeed, for thequantification of cholesterol and cholesterol esters with have the kits ab65359and ab102515 available.ab65359uses both colorimetric and fluorometric detection, whereas ab102515 uses only colorimetric d...

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    I have many samples to prepare and I'd like to measure them all together. What is a good stopping point during this protocol? Also, the protocol states "For cells or tissue samples, extract 106 cells or 10 mg tissue with 200 µl CHCl3:IPA:NP-40 (7:11:0....

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    1) A good stopping point would be after the samples are dried "(store at -20 °C until ready to proceed): Transfer the liquid phase to a new tube and air dry at 50°C then place samples under vacuum for 30 min to remove any remaining solvent." 2) Usually...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"