Anti-Choline Acetyltransferase antibody - Neuronal Marker (ab6168)
Overview
- Product nameAnti-Choline Acetyltransferase antibody - Neuronal MarkerSee all Choline Acetyltransferase primary antibodies ...
- DescriptionRabbit polyclonal to Choline Acetyltransferase - Neuronal Marker
- SpecificityExtensive immunohistochemistry indicates localisation to cholinergic nervous system only. Cross reactivity: This antibody is known to react with rat and guinea pig. Cross reactivity with other species has not been tested yet.
- Tested applicationsICC/IF, ELISA, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Rat, Guinea pig
Predicted to work with: Chicken, Human, Non Human PrimatesDoes not react with
Sheep - Immunogen
Synthetic peptide: H-GLFSSYRLPGHTQDTLVAQKSS conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 168-189 of Choline Acetyltransferase.
- General notesThis antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
- PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. See Abreview. |
| ELISA | ELISA: 1/1000. |
| IHC-P | IHC-P: Use at an assay dependent dilution. PubMed: 19422789 |
| IHC-Fr | IHC-Fr: 1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde (4% formaldehyde with or without 15% picric acid is a very good fixative, it should also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in 30% sucrose (at +4C). Use on free floating sections for best results.This antibody does not work with glutaraldehyde fixation. |
Target
- FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
- Involvement in diseaseDefects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
- Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
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Database links
- Entrez Gene: 1103 Human
- Entrez Gene: 12647 Mouse
- Entrez Gene: 290567 Rat
- Omim: 118490 Human
- SwissProt: P28329 Human
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 302002 Human
- Unigene: 442817 Mouse
- Unigene: 45116 Rat
see all
Target information above from: UniProt accession
P28329
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Acetyl CoA choline O acetyltransferase antibodyacetyl CoA:choline O acetyltransferase antibodyChAT antibody
- CHOACTase antibodyCholine acetylase antibodycholine acetyltransferase antibodyCholine O acetyltransferase antibodyCholine O acetyltransferase antibodyCholine O-acetyltransferase antibodyCLAT_HUMAN antibodyCMS1A antibodyCMS1A2 antibodyEC 2.3.1.6 antibodyOTTHUMP00000019583 antibodyOTTHUMP00000019584 antibody
see all
Anti-Choline Acetyltransferase antibody - Neuronal Marker images
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Immunohistochemistry (Frozen sections) - Choline Acetyltransferase antibody - Neuronal Marker (ab6168)Marcin Arciszewski, Org unknown, Country unknownab6168 staining rat stomach tissue sections by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde. The primary antibody was diluted 1/200 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal (IgG whole molecule) to rabbit IgG, diluted 1/400 was used as secondary.
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Immunocytochemistry/ Immunofluorescence - Choline Acetyltransferase antibody - Neuronal Marker (ab6168)This image is courtesy of an Abreview submitted by Dr Li-Hsien Linab6168 staining cells in rat brain section by ICC/IF. Cells were PFA fixed and blocked in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 in 10% donkey serum and incubated with the sample for 24 hours at 25°C. A FITC-conjugated donkey polyclonal to rabbit IgG, diluted 1/200 was used the secondary.
References for Anti-Choline Acetyltransferase antibody - Neuronal Marker (ab6168)
This product has been referenced in:
- Tu JL et al. APOE 4 polymorphism results in early cognitive deficits in an EAE model. Biochem Biophys Res Commun 384:466-70 (2009). WB, IHC-P ; Mouse . Read more (PubMed: 19422789) »
- Masliukov PM & Timmermans JP Immunocytochemical properties of stellate ganglion neurons during early postnatal development. Histochem Cell Biol 122:201-9 (2004). Read more (PubMed: 15338227) »
