Anti-Choline Acetyltransferase antibody - Neuronal Marker (ab6168)

Overview

  • Product nameAnti-Choline Acetyltransferase antibody - Neuronal MarkerSee all Choline Acetyltransferase primary antibodies ...
  • Description
    Rabbit polyclonal to Choline Acetyltransferase - Neuronal Marker
  • SpecificityExtensive immunohistochemistry indicates localisation to cholinergic nervous system only. Cross reactivity: This antibody is known to react with rat and guinea pig. Cross reactivity with other species has not been tested yet.
  • Tested applicationsICC/IF, ELISA, IHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig
    Predicted to work with: Chicken, Human, Non Human Primates

    Does not react with

    Sheep
  • Immunogen

    Synthetic peptide: H-GLFSSYRLPGHTQDTLVAQKSS conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 168-189 of Choline Acetyltransferase.

  • General notesThis antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.

Properties

Applications

Our Abpromise guarantee covers the use of ab6168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent dilution. See Abreview.
ELISA 1/1000.
IHC-P Use at an assay dependent dilution. PubMed: 19422789
IHC-Fr 1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde (4% formaldehyde with or without 15% picric acid is a very good fixative, it should also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in 30% sucrose (at +4C). Use on free floating sections for best results.
This antibody does not work with glutaraldehyde fixation.

Target

  • FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • Involvement in diseaseDefects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement.
  • Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
  • Target information above from: UniProt accession P28329 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Acetyl CoA choline O acetyltransferase antibody
    • acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Anti-Choline Acetyltransferase antibody - Neuronal Marker images

  • ab6168 staining rat stomach tissue sections by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde. The primary antibody was diluted 1/200 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal (IgG whole molecule) to rabbit IgG, diluted 1/400 was used  as secondary. 

    See Abreview

  • ab6168 staining cells in rat brain section by ICC/IF. Cells were PFA fixed and blocked in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 in 10% donkey serum and incubated with the sample for 24 hours at 25°C. A FITC-conjugated donkey polyclonal to rabbit IgG, diluted 1/200 was used the secondary.

    See Abreview

References for Anti-Choline Acetyltransferase antibody - Neuronal Marker (ab6168)

This product has been referenced in:
  • Tu JL  et al. APOE 4 polymorphism results in early cognitive deficits in an EAE model. Biochem Biophys Res Commun 384:466-70 (2009). WB, IHC-P ; Mouse . Read more (PubMed: 19422789) »
  • Masliukov PM & Timmermans JP Immunocytochemical properties of stellate ganglion neurons during early postnatal development. Histochem Cell Biol 122:201-9 (2004). Read more (PubMed: 15338227) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.25 % Triton-x ind 0.1M PB
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 10 2013

Thank you for your reply.

We have not validated ab6168 for use on perfused-fixed frozen sections (IHC-FoFr), and it would not be guaranteed for use in that application. The alternative antibody ab18736 is known to work well on perfused-fixed ...

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Thank you for your email. I am sorry that this antibody is giving a weak signal. Could you please clarify a few points of your protocol?

1) What type of samples were tested? Were these rat brain sections or some other type of tissue? How long...

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Thank you for contacting Abcam. I am sorry that you are having problems with ab6168 in IHC-P. I found the following reference that used the antibody successfully in IHC-P on mouse tissue: Tu JL et al. APOE 4 polymorphism results in early cognitive defi...

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Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use...

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Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Stomach)
Specification Stomach
Fixative Paraformaldehyde
Permeabilization No
Username

Prof. Marcin Arciszewski

Verified customer

Submitted Oct 27 2008

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Li-Hsien Lin

Verified customer

Submitted Oct 10 2008

Application Immunohistochemistry (Frozen sections)
Sample Sheep Tissue sections (pancreas)
Specification pancreas
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1
Username

Prof. Marcin Arciszewski

Verified customer

Submitted Feb 22 2006

I'm afraid we do not currently sell this kit in our catalogue, I would recommend to look on Biocompare.com, this may help you find a suitable supplier. I look forward to hearing how you get on with those changes. As I mentioned before the two refere...

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Thank you for your protocol details, I think there are several possible changes you can make to your protocol to get better signal. However I must emphasise that immunohistochemistry on formalin fixed paraffin embedded sections has never been tested an...

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